• Immunofluorescence analysis of U-2 OS cells using ETFB Rabbit polyclonal antibody (STJ118483) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ETFB Rabbit polyclonal antibody (STJ118483) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ETFB Rabbit polyclonal antibody (STJ118483) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ETFB Rabbit polyclonal antibody (STJ118483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ETFB antibody (80-230) (STJ118483)

SKU:
STJ118483

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ETFB (80-230) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ETFB
Gene ID: 2109
Uniprot ID: ETFB_HUMAN
Immunogen Region: 80-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-230 of human ETFB (NP_001976.1).
Immunogen Sequence: AMGADRGIHVEVPPAEAERL GPLQVARVLAKLAEKEKVDL VLLGKQAIDDDCNQTGQMTA GFLDWPQGTFASQVTLEGDK LKVEREIDGGLETLRLKLPA VVTADLRLNEPRYATLPNIM KAKKKKIEVIKPGDLGVDLT SKLSVISVEDP
Tissue Specificity Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
Post Translational Modifications Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from acyl-CoA dehydrogenases.
Function Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purely structural role.
Protein Name Electron Transfer Flavoprotein Subunit Beta
Beta-Etf
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-8876725
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Electron Transfer Flavoprotein Subunit Beta antibody
Anti-Beta-Etf antibody
Anti-ETFB antibody
Anti-FP585 antibody

Information sourced from Uniprot.org

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