• Western blot analysis of extracts of various cell lines, using EWSR1 rabbit monoclonal antibody (STJ11102513) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded human breast cancer tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded human breast tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded human thyroid tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded human tonsil tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse colon tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse heart tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse kidney tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse liver tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse lung tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded rat colon tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EWSR1 in paraffin-embedded rat spleen tissue using EWSR1 rabbit monoclonal antibody (STJ11102513) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using EWSR1 rabbit monoclonal antibody (STJ11102513) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using EWSR1 rabbit monoclonal antibody (STJ11102513) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using EWSR1 rabbit monoclonal antibody (STJ11102513) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EWSR1 antibody (1-189) [S3MR] (STJ11102513)

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STJ11102513

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-EWSR1 (1-189) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EWSR1
Gene ID: 2130
Uniprot ID: EWS_HUMAN
Immunogen Region: 1-189
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human EWSR1 (Q01844).
Immunogen Sequence: MASTDYSTYSQAAAQQGYSA YTAQPTQGYAQTTQAYGQQS YGTYGQPTDVSYTQAQTTAT YGQTAYATSYGQPPTGYTTP TAPQAYSQPVQGYGTGAYDT TTATVTTTQASYAAQSAYGT QPAYPAYGQQPAATAPTRPQ DGNKPTETSQPQSSTGGYNQ PSLGYGQSNYSYPQVPGSYP MQPVTAPPS
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated.calmodulin-binding inhibits phosphorylation of Ser-266. Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8.
Function Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Protein Name Rna-Binding Protein Ews
Ews Oncogene
Ewing Sarcoma Breakpoint Region 1 Protein
Cellular Localisation Nucleus
Cytoplasm
Cell Membrane
Relocates From Cytoplasm To Ribosomes Upon Ptk2b/Fak2 Activation
Alternative Antibody Names Anti-Rna-Binding Protein Ews antibody
Anti-Ews Oncogene antibody
Anti-Ewing Sarcoma Breakpoint Region 1 Protein antibody
Anti-EWSR1 antibody
Anti-EWS antibody

Information sourced from Uniprot.org

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