Anti-eRF3A/GSPT1 antibody [SRM14826] (STJ11109556)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal eRF3A/GSPT1 antibody for use in WB, IF, ICC, IF-P, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IF-P/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14826 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:10000-1:60000IF/ICC, 1:200-1:800IF-P, 1:200-1:800IHC-P, 1:500-1:2000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GSPT1 |
| Gene ID | 2935 |
| Uniprot ID | ERF3A_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
Additional Info
| Function | GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. |
| Protein Name | Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3aEukaryotic Peptide Chain Release Factor Subunit 3aErf3aG1 To S Phase Transition Protein 1 Homolog |
| Database Links | Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3a antibodyAnti-Eukaryotic Peptide Chain Release Factor Subunit 3a antibodyAnti-Erf3a antibodyAnti-G1 To S Phase Transition Protein 1 Homolog antibodyAnti-GSPT1 antibodyAnti-ERF3A antibody |
Information sourced from Uniprot.org