• Immunofluorescence analysis of HeLa cells using ETS1 Rabbit polyclonal antibody (STJ11103852) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A549 cells using ETS1 Rabbit polyclonal antibody (STJ11103852). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ETS1 in paraffin-embedded human esophagus using ETS1 Rabbit polyclonal antibody (STJ11103852) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ETS1 in paraffin-embedded human liver damage using ETS1 Rabbit polyclonal antibody (STJ11103852) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ETS1 in paraffin-embedded human lung cancer using ETS1 Rabbit polyclonal antibody (STJ11103852) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse kidney, using ETS1 Rabbit polyclonal antibody (STJ11103852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ETS1 antibody (1-260) (STJ11103852)

SKU:
STJ11103852

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein C-Ets-1 (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ETS1
Gene ID: 2113
Uniprot ID: ETS1_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ETS1 (NP_005229.1).
Immunogen Sequence: MKAAVDLKPTLTIIKTEKVD LELFPSPDMECADVPLLTPS SKEMMSQALKATFSGFTKEQ QRLGIPKDPRQWTETHVRDW VMWAVNEFSLKGVDFQKFCM NGAALCALGKDCFLELAPDF VGDILWEHLEILQKEDVKPY QVNGVNPAYPESRYTSDYFI SYGIEHAQCVPPSEFSEPSF ITESYQTLHPISSEELLSLK YENDYPSVILRDPLQTDTLQ NDYFAIKQEVVTPDNMCMG
Tissue Specificity Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.
Post Translational Modifications Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2.which inhibits transcriptional activity. Ubiquitinated.which induces proteasomal degradation. Phosphorylation at Ser-251, Ser-282 and Ser-285 by CaMK2/CaMKII in response to calcium signaling decreases affinity for DNA: an increasing number of phosphoserines causes DNA-binding to become progressively weaker.
Function Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion. Isoform Ets-1 p27: Acts as a dominant-negative for isoform c-ETS-1A.
Protein Name Protein C-Ets-1
P54
Database Links Reactome: R-HSA-2559585
Cellular Localisation Nucleus
Cytoplasm
Delocalizes From Nucleus To Cytoplasm When Coexpressed With Isoform Ets-1 P27
Alternative Antibody Names Anti-Protein C-Ets-1 antibody
Anti-P54 antibody
Anti-ETS1 antibody
Anti-EWSR2 antibody

Information sourced from Uniprot.org

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