Immunocytochemistry Antibodies | St John's Laboratory

Immunocytochemistry analysis of Smad4 (green) in Hela using Smad4 antibody, and DAPI (blue).

Anti-SMAD4 antibody (316-552 aa) [R05-1I7] (STJA0012592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£264.50

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Western blot analysis of lysates from HCT116 cells, using Smad4 Rabbit pAb (STJ27624) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SMAD4 antibody (320-552) (STJ27624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SMAD5 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-SMAD5 antibody (244-262 aa) [R07-2A5] (STJA0011975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£264.50

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Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.

Anti-SMAD5 antibody (C-Term) [4B10-B10-B6] (STJ99081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/FC

Reactivity :

Human

£149.00 - £360.00

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Anti-SMAD7 antibody (N-Term) (STJA0005586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

£319.50

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Immunofluorescence analysis of L929 cells using SMAP1 Rabbit polyclonal antibody (STJ119790) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SMAP1 antibody (180-250) (STJ119790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using SMARCA1 antibody (STJ112286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SMARCA1 antibody (1-200) (STJ112286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunocytochemistry analysis of SMARCA2 (green) in 293 using SMARCA2 antibody, and DAPI (blue).

Anti-SMARCA2 antibody (62-77 aa) [R02-3E2] (STJA0012593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£264.50

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Anti-SMARCA2/BRM antibody [SRM14623] (STJ11109353)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ChIP/ELISA

Reactivity :

Human

£0.00

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Western blot analysis of extracts of various cell lines, using BRG1/SMARCA4 antibody (STJ11101695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SMARCA4 antibody (200-300) [S5MR] (STJ11101695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of HeLa cells using BRG1/BRG1/SMARCA4 antibody (STJ113555).

Anti-SMARCA4 antibody (30-130) (STJ113555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g BRG1/SMARCA4 antibody (STJ25612). Western blot was performed from the immunoprecipitate using BRG1/SMARCA4 antibody (STJ25612) at a dilution of 1:1000.

Anti-SMARCA4 antibody (680-770) (STJ25612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Confocal imaging of C2C12 cells using SMARCA5/SNF2H Rabbit monoclonal antibody (STJ11101895, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-SMARCA5 antibody (953-1052) [S5MR] (STJ11101895)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of A549 cells using SMARCAD1 antibody (STJ28413). Blue: DAPI for nuclear staining.

Anti-SMARCAD1 antibody (1-300) (STJ28413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SMARCAD1 antibody [SRM14936] (STJ11109666)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g SMARCB1/SNF5 antibody (STJ28334). Western blot was performed from the immunoprecipitate using SMARCB1/SNF5 antibody (STJ28334) at a dilution of 1:1000.

Anti-SMARCB1 antibody (50-150) (STJ28334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SMARCB1/SNF5 antibody [SRM13306] (STJ11108036)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Chromatin immunoprecipitation analysis of extracts of MCF7 beta-estradiol cells, using SMARCC1/BAF155 antibody (STJ11102030) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-SMARCC1 antibody (700-800) [S0MR] (STJ11102030)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunoprecipitation analysis of 150 Mu g extracts of Jurkat cells using 3 Mu g BAF57/BAF57/SMARCE1 antibody (STJ115316). Western blot was performed from the immunoprecipitate using BAF57/BAF57/SMARCE1 antibody (STJ115316) at a dilution of 1:1000.

Anti-SMARCE1 antibody (1-411) (STJ115316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of U-2 OS cells using BAF57/SMARCE1 Rabbit monoclonal antibody (STJ11101955) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMARCE1 antibody (2-100) [S5MR] (STJ11101955)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Confocal imaging of HeLa cells using SMC1 Rabbit monoclonal antibody (STJ11102132, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-SMC1A antibody (1134-1233) [S2MR] (STJ11102132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot detection of SMC1A (C-term) in MOLT-4, U87 MG and HelaNE cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.

Anti-SMC1A antibody (C-Term) [4C5-C8-A11] (STJ99054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£149.00 - £360.00

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Anti-SMC3 antibody [SRM13066] (STJ11107796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of U-2 OS cells using SMC4 Rabbit monoclonal antibody (STJ11101897) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMC4 antibody (1189-1288) [S7MR] (STJ11101897)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of MCF-7 cells using SMCHD1 antibody (STJ29294). Blue: DAPI for nuclear staining.

Anti-SMCHD1 antibody (1756-2005) (STJ29294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa survival motor neuron (SMN) protein.

Anti-SMN antibody [4B7] (STJA0003856)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£454.50

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Immunofluorescence analysis of U2OS cells using SMN2 Rabbit polyclonal antibody (STJ114382) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SMN2 antibody (1-197) (STJ114382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using SMN2 antibody (STJ114393) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMN2 antibody (1-197) (STJ114393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g SMN2 antibody (STJ29863). Western blot was performed from the immunoprecipitate using SMN2 antibody (STJ29863) at a dilution of 1:1000.

Anti-SMN2 antibody (1-197) (STJ29863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Smoothelin antibody [R4A] (STJ16100219)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/IHC-F/IHC-P/WB

Reactivity :

Human/Dog/Chicken/Feline/Monkey/Swine

£629.50

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Immunofluorescence analysis of HeLa cells using SMPD1/ ASM Rabbit polyclonal antibody (STJ28826). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SMPD1 antibody (70-340) (STJ28826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of NIH-3T3 cells using SMPD1/ ASM Polyclonal Antibody (STJ118698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMPD1 antibody (70-631) (STJ118698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SMPD3 antibody (C-Term) (STJA0005587)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Immunofluorescence analysis of L929 cells using SMPD4 Rabbit polyclonal antibody (STJ117668) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SMPD4 antibody (600-800) (STJ117668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-SMPD4 antibody (C-Term) (STJA0005588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£367.50

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Immunoprecipitation analysis of 300 Mu g extracts of Mouse lung using 3 Mu g SMURF2 antibody (STJ11101803). Western blot was performed from the immunoprecipitate using SMURF2 antibody (STJ11101803) at a dilution of 1:1000.

Anti-SMURF2 antibody (201-290) [S3MR] (STJ11101803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunofluorescence analysis of MCF-7 cells using SMYD1 antibody (STJ26205). Blue: DAPI for nuclear staining.

Anti-SMYD1 antibody (1-350) (STJ26205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using SMYD2 antibody (STJ28557). Blue: DAPI for nuclear staining.

Anti-SMYD2 antibody (1-270) (STJ28557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of L929 cells using SMYD3 Rabbit polyclonal antibody (STJ116727) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SMYD3 antibody (50-150) (STJ116727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SMYD3 antibody [SRM13248] (STJ11107978)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g SMYD4 antibody (STJ116177). Western blot was performed from the immunoprecipitate using SMYD4 antibody (STJ116177) at a dilution of 1:500. ____Detection: ECL Enhanced Kit. _Exposure time: 20s.

Anti-SMYD4 antibody (1-260) (STJ116177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of HeLa, using SMYD4 rabbit polyclonal antibody (STJ29449) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SMYD4 antibody (1-260) (STJ29449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of A549 cells using SMYD5 antibody (STJ27952). Blue: DAPI for nuclear staining.

Anti-SMYD5 antibody (300-400 aa) (STJ27952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of 293F, using Snail antibody (STJ113375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SNAI1 antibody (200-267) (STJ113375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Snail antibody (STJ29909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SNAI1 antibody (200-267) (STJ29909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Snail antibody (STJ27533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SNAI1 antibody (C-Term) (STJ27533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Snail antibody [SRM13419] (STJ11108149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

£0.00

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Immunofluorescence analysis of mouse brain using SNAP25 Rabbit polyclonal antibody (STJ25637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SNAP25 antibody (1-206) (STJ25637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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