• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody (STJ11102119) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunofluorescence analysis of U-2 OS cells using [KO Validated] MTA2 Rabbit monoclonal antibody (STJ11102119) at dilution of 1 : 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MTA2 in paraffin-embedded human colon using MTA2 Rabbit monoclonal antibody (STJ11102119) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and MTA2 knockout (KO) 293T cells, using [KO Validated] MTA2 Rabbit monoclonal antibody (STJ11102119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using MTA2 Rabbit monoclonal antibody (STJ11102119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-MTA2 antibody (569-668) [S9MR] (STJ11102119)

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STJ11102119

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MTA2 (569-668) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTA2
Gene ID: 9219
Uniprot ID: MTA2_HUMAN
Immunogen Region: 569-668
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 569-668 of human MTA2 (O94776).
Immunogen Sequence: GVPFSANGRPLASGIRSSSQ PAAKRQKLNPADAPNPVVFV ATKDTRALRKALTHLEMRRA ARRPNLPLKVKPTLIAVRPP VPLPAPSHPASTNEPIVLED
Tissue Specificity Widely expressed.
Function May function as a transcriptional coregulator. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin.
Protein Name Metastasis-Associated Protein Mta2
Metastasis-Associated 1-Like 1
Mta1-L1 Protein
P53 Target Protein In Deacetylase Complex
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-427389
Reactome: R-HSA-6804758
Reactome: R-HSA-73762
Reactome: R-HSA-8943724
Reactome: R-HSA-9679191
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Metastasis-Associated Protein Mta2 antibody
Anti-Metastasis-Associated 1-Like 1 antibody
Anti-Mta1-L1 Protein antibody
Anti-P53 Target Protein In Deacetylase Complex antibody
Anti-MTA2 antibody
Anti-MTA1L1 antibody
Anti-PID antibody

Information sourced from Uniprot.org

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