Immunocytochemistry Antibodies | St John's Laboratory

Western blot analysis of extracts of Rat placenta, using PAI-1/Serpin E1 antibody (STJ27967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SERPINE1 antibody (24-199) (STJ27967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PEDF/SERPINF1 Rabbit polyclonal antibody (STJ25489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-SERPINF1 antibody (141-240) (STJ25489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of Hep G2 cells using SERPING1 Rabbit polyclonal antibody (STJ114281) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SERPING1 antibody (201-500) (STJ114281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells using SERPING1 Rabbit polyclonal antibody (STJ25490) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-SERPING1 antibody (201-500) (STJ25490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using HSP47/SERPINH1 Rabbit polyclonal antibody (STJ11103839) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SERPINH1 antibody (139-418) (STJ11103839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Confocal imaging of NIH/3T3 cells using HSP47/SERPINH1 Rabbit monoclonal antibody (STJ11101393, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-SERPINH1 antibody (319-418) [S3MR] (STJ11101393)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of paraffin-embedded mouse pancreas using SERPINI2 Rabbit polyclonal antibody (STJ113329) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SERPINI2 antibody (56-405) (STJ113329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using SET/TAF1 Rabbit mAb (STJ11102445) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-SET antibody (200-290) [S5MR] (STJ11102445)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of A-549 cells using SETBP1 Rabbit polyclonal antibody (STJ29292). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-SETBP1 antibody (1-242) (STJ29292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of mouse spleen, using SETD2 antibody (STJ25493) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SETD2 antibody (2475-2564) (STJ25493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SETD2 antibody [SRM13283] (STJ11108013)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemistry analysis of paraffin-embedded mouse brain using SETD5 Rabbit polyclonal antibody (STJ29443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-SETD5 antibody (1054-1153) (STJ29443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of MCF-7 cells using SETD5 antibody (STJ116075). Blue: DAPI for nuclear staining.

Anti-SETD5 antibody (520-740) (STJ116075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and SETD7 knockout (KO) 293T cells, using [KO Validated] SETD7 Rabbit pAb (STJ112026) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-SETD7 antibody (1-366) (STJ112026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Confocal immunofluorescence analysis of U2OS cells using SF3A3 Polyclonal Antibody (STJ25497) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SF3A3 antibody (292-501) (STJ25497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using SF3B1 Rabbit mAb (STJ11103676) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SF3B1 antibody (1-100) [S6MR] (STJ11103676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using SF3B1 antibody (STJ118246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SF3B1 antibody (1-144) (STJ118246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A-549 cells using SF3B2 antibody (STJ28148).

Anti-SF3B2 antibody (616-895) (STJ28148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using SF3B3/SAP130 Rabbit mAb (STJ11102510) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-SF3B3 antibody (1101-1217) [S0MR] (STJ11102510)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunocytochemistry analysis of SF3B3 (green) in Hela using SF3B3 antibody, and DAPI (blue)

Anti-SF3B3 antibody (1184-1201 aa) [R07-2K8] (STJA0011953)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£264.50

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Immunoflourescence of HeLa cells labeled with anti-SAP49 (STJA0003775, red, 1:1000) and anti-vimentin (STJA0003823, green, 1:10,000). The blue is DAPI staining nuclear DNA.

Anti-SF3B4 antibody (Full Length) [3A1] (STJA0003775)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

£454.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g SFPQ antibody (STJ25501). Western blot was performed from the immunoprecipitate using SFPQ antibody (STJ25501) at a dilution of 1:1000.

Anti-SFPQ antibody (578-707) (STJ25501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using SFPQ antibody (STJ11101884) at 1:200 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SFPQ antibody (600-707) [S4MR] (STJ11101884)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunocytochemistry analysis of SFPQ (green) in 293T using SFPQ antibody, and DAPI (blue).

Anti-SFPQ antibody (694-707 aa) [R07-9K6] (STJA0011954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£264.50

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Immunofluorescence analysis of HeLa cells using SFRP4 antibody (STJ28492). Blue: DAPI for nuclear staining.

Anti-SFRP4 antibody (19-346) (STJ28492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of NIH/3T3 cells using SFRP4 Rabbit monoclonal antibody (STJ11102014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-SFRP4 antibody (201-300) [S4MR] (STJ11102014)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of mouse pancreas using SFRP5 Polyclonal Antibody (STJ119145) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SFRP5 antibody (30-317) (STJ119145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded mouse lung using SFTPC Rabbit polyclonal antibody (STJ25509) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SFTPC antibody (59-197) (STJ25509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SFTPC antibody (91-190 aa) [SRM5344] (STJ11107253)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Immunofluorescence analysis of C6 cells using alpha Sarcoglycan (alpha Sarcoglycan (SGCA) ) Rabbit polyclonal antibody (STJ116232) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SGCA antibody (60-290) (STJ116232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using Delta-sarcoglycan (Delta-sarcoglycan (SGCD) ) antibody (STJ115314). Blue: DAPI for nuclear staining.

Anti-SGCD antibody (61-290) (STJ115314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using SGK1 Rabbit polyclonal antibody (STJ25513) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SGK1 antibody (172-431) (STJ25513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using SGMS1 Rabbit polyclonal antibody (STJ117204) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SGMS1 antibody (1-134) (STJ117204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U20S cells using SGOL1 Rabbit polyclonal antibody (STJ118614) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SGOL1 antibody (10-100) (STJ118614)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of SGPL1 in paraffin-embedded Human lung cancer tissue using SGPL1 Rabbit pAb (STJ11107092) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-SGPL1 antibody (301-400 aa) (STJ11107092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using SGPL1 rabbit polyclonal antibody (STJ118190) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SGPL1 antibody (60-390) (STJ118190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SGPL1 antibody [SRM14931] (STJ11109661)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-SGSH antibody [PT0814R] (STJA0039375)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ICC

Reactivity :

Human

£0.00

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Immunofluorescence analysis of A-549 cells using SGTA antibody (STJ29445). Blue: DAPI for nuclear staining.

Anti-SGTA antibody (1-120) (STJ29445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using SH2B1 antibody (STJ27510). Blue: DAPI for nuclear staining.

Anti-SH2B1 antibody (360-520) (STJ27510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using SH2D1A Rabbit polyclonal antibody (STJ25517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SH2D1A antibody (1-128) (STJ25517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using SH3BGRL Rabbit polyclonal antibody (STJ119622) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SH3BGRL antibody (1-114) (STJ119622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ70507 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-SH3BP1 antibody (C-Term) (STJ70507)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/ICC/IF

Reactivity :

Human

£312.50

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Immunofluorescence analysis of HeLa cells using SH3GL2 antibody (STJ27668). Blue: DAPI for nuclear staining.

Anti-SH3GL2 antibody (1-270) (STJ27668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of A549 cells using Bif-1 Rabbit polyclonal antibody (STJ29184). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SH3GLB1 antibody (1-365) (STJ29184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from HeLa cells, using SH3PXD2A Rabbit pAb (STJ11102477). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SH3PXD2A antibody (806-1105) (STJ11102477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-Shade antibody (1-50) (STJ503002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IHC/IF/WB

Reactivity :

D.melanogaster

£427.50

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Anti-Shade antibody (1-50) {Biotin} (STJ503004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IHC/IF/WB

Reactivity :

D.melanogaster

£528.50

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