• Immunofluorescence analysis of NIH-3T3 cells using MKNK1 Rabbit polyclonal antibody (STJ11100383) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using MKNK1 Rabbit polyclonal antibody (STJ11100383) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using MKNK1 Rabbit polyclonal antibody (STJ11100383) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat fallopian tube using MKNK1 Rabbit polyclonal antibody (STJ11100383) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using MKNK1 Rabbit polyclonal antibody (STJ11100383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-MKNK1 antibody (374-465) (STJ11100383)

SKU:
STJ11100383

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MKNK1 (374-465) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MKNK1
Gene ID: 8569
Uniprot ID: MKNK1_HUMAN
Immunogen Region: 374-465
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 374-465 of human MKNK1 (NP_003675.2).
Immunogen Sequence: VQGQAPEKGLPTPQVLQRNS STMDLTLFAAEAIALNRQLS QHEENELAEEPEALADGLCS MKLSPPCKSRLARRRALAQA GRGEDRSPPTAL
Tissue Specificity Ubiquitous.
Post Translational Modifications Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.
Function May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Protein Name Map Kinase-Interacting Serine/Threonine-Protein Kinase 1
Map Kinase Signal-Integrating Kinase 1
Mapk Signal-Integrating Kinase 1
Mnk1
Database Links Reactome: R-HSA-1295596
Cellular Localisation Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Nucleus
Alternative Antibody Names Anti-Map Kinase-Interacting Serine/Threonine-Protein Kinase 1 antibody
Anti-Map Kinase Signal-Integrating Kinase 1 antibody
Anti-Mapk Signal-Integrating Kinase 1 antibody
Anti-Mnk1 antibody
Anti-MKNK1 antibody
Anti-MNK1 antibody

Information sourced from Uniprot.org

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