• Western blot analysis of extracts of various cell lines, using MLF1 antibody (STJ110318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of A549 cells using MLF1 antibody (STJ110318).
  • Immunofluorescence analysis of A549 cells using MLF1 antibody (STJ110318).
  • Western blot analysis of extracts of various cell lines, using MLF1 antibody (STJ110318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MLF1 antibody (1-268) (STJ110318)

SKU:
STJ110318

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MLF1 (1-268) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MLF1
Gene ID: 4291
Uniprot ID: MLF1_HUMAN
Immunogen Region: 1-268
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human MLF1 (NP_071888.1).
Immunogen Sequence: MFRMLNSSFEDDPFFSESIL AHRENMRQMIRSFSEPFGRD LLSISDGRGRAHNRRGHNDG EDSLTHTDVSSFQTMDQMVS NMRNYMQKLERNFGQLSVDP NGHSFCSSSVMTYSKIGDEP PKVFQASTQTRRAPGGIKET RKAMRDSDSGLEKMAIGHHI HDRAHVIKKSKNKKTGDEEV NQEFINMNESDAHAFDEEWQ SEVLKYKPGRHNLGNTRMRS VGHENPGSRELKRREKPQQ
Tissue Specificity Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
Post Translational Modifications Phosphorylation is required for binding to YWHAZ.
Function Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Protein Name Myeloid Leukemia Factor 1
Myelodysplasia-Myeloid Leukemia Factor 1
Cellular Localisation Cytoplasm
Nucleus
Cell Projection
Cilium
Cytoskeleton
Cilium Basal Body
Shuttles Between The Cytoplasm And Nucleus
Alternative Antibody Names Anti-Myeloid Leukemia Factor 1 antibody
Anti-Myelodysplasia-Myeloid Leukemia Factor 1 antibody
Anti-MLF1 antibody

Information sourced from Uniprot.org

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