ELISA Antibodies | St John's Laboratory

Western blot analysis of lysates from rat pancreas, using ASCC1 Rabbit polyclonal antibody (STJ118277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ASCC1 antibody (1-180) (STJ118277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASCC2 Rabbit polyclonal antibody (STJ115734) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASCC2 antibody (488-757) (STJ115734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using ASCC3 Rabbit pAb (STJ110267) at 1:800 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 50s.

Anti-ASCC3 antibody (1-111) (STJ110267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunohistochemistry analysis of ASCL1 antibody in paraffin-embedded human brain tissue.

Anti-ASCL1 antibody (109-158 aa) (STJ91732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using AscL1 Rabbit polyclonal antibody (STJ118012) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AscL1 antibody (150-236) (STJ118012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200

Anti-ASCL1 antibody (190-236 aa) (STJ98708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ73536 (1µg/ml) staining of Mouse (A) and Rat (B) Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-ASCL1 antibody (79-91) (STJ73536)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Cow/Mouse/Rat/Dog

$421.88

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Anti-Ascl1a antibody (Internal) (STJ71255)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Zebrafish

$261.23

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASCL2 antibody (1-80 aa) (STJ94020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Ascl2 antibody (185-263 aa) (STJ11106594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ72471 (0. 1µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Ascl3-Mouse antibody (Internal) (STJ72471)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Cow

$421.88

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using ASCL4 antibody (STJ116649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASCL4 antibody (1-70) (STJ116649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from mouse pancreas, using ASF1A Rabbit pAb (STJ28611) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 20s.

Anti-ASF1A antibody (1-204) (STJ28611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ASF1A antibody (100-204) (STJ116719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various cells using Asf1a Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ASF1A antibody (121-170 aa) (STJ91733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot with Anti-ASF1A Antibody (STJ500171) of rat muscle membranes at 1:500 dilution in buffer.

Anti-ASF1A antibody (60-120) (STJ500171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-ASF1A antibody (60-120) {Biotin} (STJ500172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-ASF1A antibody (60-120) {FITC} (STJ500173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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STJ71364 (1µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASF1A antibody (C-Term) (STJ71364)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

$421.88

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STJ71180 (0. 01µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASF1A/HSPC146 antibody (Internal) (STJ71180)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

$421.88

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Western blot analysis of lysates from K562 cells, using ASF1B Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ASF1B antibody (101-150 aa) (STJ91734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ASF1B antibody (STJ28594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASF1B antibody (63-202) (STJ28594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-ASGPR antibody [8D7] (STJ16100783)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/ELISA/IF/WB

Reactivity :

Rat/Human

$1,083.38

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ASGR1 antibody (STJ28951) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASGR1 antibody (1-150) (STJ28951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded mouse liver using ASGR1 Rabbit polyclonal antibody (STJ115244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ASGR1 antibody (62-609) (STJ115244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASGR2 Rabbit polyclonal antibody (STJ115884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ASGR2 antibody (131-287) (STJ115884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded Mouse liver tissue using ASGR2 Rabbit polyclonal antibody (STJ28203) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ASGR2 antibody (58-287) (STJ28203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ASH1L antibody (2074-2293 aa) (STJ11106636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-ASH2L antibody (235-534) (STJ27345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

$151.88 - $708.08

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Confocal imaging of paraffin-embedded Mouse colon tissue using ASH2L Rabbit monoclonal antibody (STJ11102184, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ASH2L antibody (529-628) [S4MR] (STJ11102184)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various lysates using ASH2L Rabbit pAb (STJ116754) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ASH2L antibody (81-280) (STJ116754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of mouse-brain, mouse-spinal-cord lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-ASIC1 antibody (220-280 aa) (STJ98706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IHC-F/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ASIC1 antibody (70-360 aa) (STJ11105986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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STJ73108 (0. 3µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ASIC1 antibody (C-Term) (STJ73108)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$421.88

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Western blot analysis of various lysates using ASIC2 Rabbit polyclonal antibody (STJ112520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ASIC2 antibody (120-400) (STJ112520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot with Anti-ASIC2 Antibody (STJ500174) of mouse kidney at 1:1000 dilution in buffer.

Anti-ASIC2 antibody (450-512) (STJ500174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-ASIC2 antibody (450-512) {Biotin} (STJ500175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-ASIC2 antibody (450-512) {FITC} (STJ500176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Western blot analysis of HepG2 cells using ASIC3 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ASIC3 antibody (191-240 aa) (STJ96565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ASIC3 antibody (STJ112306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASIC3 antibody (62-320) (STJ112306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASIC5 Rabbit pAb (STJ112345) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ASIC5 antibody (140-330) (STJ112345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using ASIP antibody (STJ115245). Blue: DAPI for nuclear staining.

Anti-ASIP antibody (23-132) (STJ115245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded human gastric cancer using ASIP antibody (STJ28952) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASIP antibody (23-132) (STJ28952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Anti-ASL antibody (289-464) (STJ28440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using ASMT rabbit polyclonal antibody (STJ28612) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ASMT antibody (1-298) (STJ28612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 293 cells, using ASMT Rabbit polyclonal antibody (STJ113419). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ASMT antibody (76-298) (STJ113419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunoprecipitation analysis of 200 Mu g extracts of SW620 cells using 1 Mu g ASMTL antibody (STJ29101). Western blot was performed from the immunoprecipitate using ASMTL antibody (STJ29101) at a dilution of 1:500.

Anti-ASMTL antibody (1-290) (STJ29101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using ASNA1 Rabbit polyclonal antibody (STJ22700) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ASNA1 antibody (1-348) (STJ22700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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