• Biotinylated STJ71128 (0. 03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Argininosuccinate synthetase 1, Biotinylated antibody (Internal) {Biotin} (STJ73211)

SKU:
STJ73211-100

$409.73
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Host: Goat
Applications: Pep-ELISA/WB/IF/IP/IHC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Argininosuccinate synthetase 1, Biotinylated (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.03µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ASS1
Gene ID: 445
Uniprot ID: ASSY_HUMAN
Immunogen Region: Internal
Accession Number: NP_000041.2
Specificity: Reported variants represent identical protein: NP_446464.1, NP_000041.2
Immunogen Sequence: ENPKNQAPPGLYTKTQD.
Post Translational Modifications Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases.
Function One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Protein Name Argininosuccinate Synthase
Citrulline--Aspartate Ligase
Database Links Reactome: R-HSA-70635
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Argininosuccinate Synthase antibody
Anti-Citrulline--Aspartate Ligase antibody
Anti-ASS1 antibody
Anti-ASS antibody

Information sourced from Uniprot.org

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