ELISA Antibodies | St John's Laboratory

Anti-ARF3 antibody (1-181) (STJ117846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ARF4 antibody (1-180 aa) (STJ11106231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-ARF4 antibody (1-180 aa) (STJ11106232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using ARF4 Rabbit polyclonal antibody (STJ117836) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARF4 antibody (1-180) (STJ117836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ72838 (0. 5µg/ml) staining of NIH3T3 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ARF4 antibody (137-150) (STJ72838)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARF4 antibody (71-120 aa) (STJ91673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-ARF4 antibody (STJ500156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-ARF4 antibody {Biotin} (STJ500157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-ARF4 antibody {FITC} (STJ500158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of lysates from wild type (WT) and ARF5 knockout (KO) 293T cells, using [KO Validated] ARF5 Rabbit polyclonal antibody (STJ11100862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARF5 antibody (1-180) (STJ11100862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using ARF5 antibody (STJ22668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ARF5 antibody (1-180) (STJ22668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARF5 antibody (40-120 aa) (STJ190524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of NIH/3T3 cells using ARF5 Rabbit monoclonal antibody (STJ11101858, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-ARF5 antibody (81-180) [S8MR] (STJ11101858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of HeLa cells using ARF6 antibody (STJ22670) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ARF6 antibody (1-175) (STJ22670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using ARF6 Rabbit polyclonal antibody (STJ22671) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ARF6 antibody (1-269) (STJ22671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARF6 antibody (30-110 aa) (STJ91674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of C6 cells using ARF6 Rabbit monoclonal antibody (STJ11101343) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ARF6 antibody (76-175) [S3MR] (STJ11101343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using ARFGAP1 Rabbit pAb (STJ114469) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-ARFGAP1 antibody (1-220) (STJ114469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (STJ29198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ARFGAP1 antibody (1-220) (STJ29198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFGAP1 antibody (171-220 aa) (STJ91671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Cow

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using ARFGAP2 Rabbit polyclonal antibody (STJ119821) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARFGAP2 antibody (242-521) (STJ119821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using ARFGAP3 Rabbit pAb (STJ110503) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ARFGAP3 antibody (217-516) (STJ110503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-ARFGAP3 antibody (280-360 aa) (STJ91672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70078 staining (2µg/ml) of mouse brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-ARFGAP3 antibody (C-Term) (STJ70078)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of various lysates using ARFGEF1 Rabbit polyclonal antibody (STJ118910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARFGEF1 antibody (220-420) (STJ118910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using BIG2 Polyclonal Antibody

Anti-ARFGEF2 antibody (1491-1540 aa) (STJ91854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ARFGEF2 antibody (220-370) (STJ117583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFIP1 antibody (271-320 aa) (STJ91675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using ARFRP1 Rabbit polyclonal antibody (STJ116523) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARFRP1 antibody (1-201) (STJ116523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded human liver cancer using Arginase 1 (ARG1) Rabbit polyclonal antibody (STJ26182) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARG1 antibody (1-322) (STJ26182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293 cells using Arginase I Polyclonal Antibody

Anti-ARG1 antibody (61-110 aa) (STJ91676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of Rat kidney, using Arginase 2 (ARG2) antibody (STJ28438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ARG2 antibody (270-354) (STJ28438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Arginase II Polyclonal Antibody

Anti-ARG2 antibody (305-354 aa) (STJ91677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human prostate cancer using Arginase 2 (Arginase 2 (ARG2) ) Rabbit monoclonal antibody (STJ11102965) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Arginase 2 antibody (255-354) [S5MR] (STJ11102965)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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STJ70719 (0. 01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase I antibody (C-Term) (STJ70719)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ71134 (0. 05µg/ml) staining of Mouse Liver (A) and Rat Liver (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase, type 1/arg1 (rat)-Rat antibody (C-Term) (STJ71134)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Mouse/Rat

£312.50

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STJ71133 (0. 01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase, type 1/ARG1 antibody (C-Term) (STJ71133)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Dog

£312.50

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Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of paraffin-embedded mouse liver using Arginase-1 Rabbit polyclonal antibody (STJ11105172) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Arginase-1 antibody (STJ11105172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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STJ71128 (0. 3µg/ml) staining of A431 (A) and (1ug/ml) NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Argininosuccinate synthetase 1 antibody (Internal) (STJ71128)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Biotinylated STJ71128 (0. 03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Argininosuccinate synthetase 1, Biotinylated antibody (Internal) {Biotin} (STJ73211)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ70389 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-ARH/LDL receptor adaptor antibody (N-Term) (STJ70389)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using RHG1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ARHGAP1 antibody (189-238 aa) (STJ91678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g ARHGAP1 antibody (STJ22672). Western blot was performed from the immunoprecipitate using ARHGAP1 antibody (STJ22672) at a dilution of 1:1000.

Anti-ARHGAP1 antibody (210-439) (STJ22672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ARHGAP10 antibody (470-570 aa) (STJ11106501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of ARHGAP11A Antibody. The lane on the right is blocked with the ARHGAP11A peptide.

Anti-ARHGAP11A antibody (471-520 aa) (STJ91679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ARHGAP11B Rabbit polyclonal antibody (STJ119014) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2s.

Anti-ARHGAP11B antibody (218-267) (STJ119014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ARHGAP12 antibody (310-390 aa) (STJ91680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ARHGAP15 antibody (STJA0004462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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