ELISA Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using 14-3-3 beta/Theta antibody (STJ11103651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-14-3-3 beta/Theta antibody (1-100) [S1MR] (STJ11103651)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from NIH/3T3 cells, using 14-3-3 beta/zeta Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-14-3-3 Beta/Zeta antibody (151-200 aa) (STJ91368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ70838 (0. 03µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-14-3-3 sigma/Stratifin antibody (Internal) (STJ70838)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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Biotinylated STJ70838 (0. 1µg/ml) staining of Mouse Skin lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-14-3-3 sigma/Stratifin, Biotinylated antibody (Internal) {Biotin} (STJ73293)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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STJ71355 (0. 03µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-14-3-3 tau/YWHAQ antibody (Internal) (STJ71355)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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The 2 NA-O-methyladenosine/ Am Rabbit polyclonal antibody (4µg, STJ11100889) are tested in Nucleotide Array against Am-1 Oligo, Am-2 Oligo and Am-4 Oligo (100nmol for each oligomer). Am-1 Oligo:biotin-CTACGGCTAA (Am) CCTTGGAm-2 Oligo:biotin-CUACGGCUAA (Am) CCUUGGAm-4 Oligo:biotin-CTACGGCTAAACCTTGG.

Anti-2 NA-O-methyl-2mA antibody (STJ11100889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Species independent

£129.50 - £642.50

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The 2‘-O-methylcytidine/Cm Rabbit polyclonal antibody (STJ11103959) are tested in Dot Blot against 2 NA-O-methylcytidine (Cm) and unmodified cytidine. Cm-3：Biotin-5 NA CGATAACCACTAGT (Cm) 3 NA Cm-4：Biotin-5 NA CGATAACCACTAGTC3 NA

Anti-2 NA-O-methylcytidine/Cm antibody (STJ11103959)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Species independent

£129.50 - £642.50

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The 2 NA-O-methylcytidine (Cm) Rabbit monoclonal antibody (STJ11104061) are tested in Dot Blot against 2 NA-O-methylcytidine and unmodified cytidine. 2 NA-O-methylcytidine ：Biotin-5 NA CGATAACCACTAGT (Cm) 3 NA unmodified cytosine：Biotin-5 NA CGATAACCACTAGTC3 NA

Anti-2 NA-O-methylcytidine/Cm antibody [S4061RM] (STJ11104061)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Species independent

£146.50 - £642.50

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Anti-2019-covid N protein antibody (STJA0010175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

£48.00 - £252.00

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Anti-2019-covid N protein antibody (STJA0010176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

£48.00 - £252.00

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Western blot analysis of recombinant SARS-COV-2 N Protein, using SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free (STJ11101210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 ng/10 ng/5 ng/2 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-2019-nCoV antibody [S0MR] (STJ11101210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IF/ICC/ELISA

Reactivity :

SARS-CoV-2

£129.50 - £574.50

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Western blot analysis of lysates from control 293T and SARS-CoV-2 Spike S1-293T trasfected cells, using SARS-CoV-2 Spike S1 mAb, BSA and glycerol free (STJ11101211) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-2019-nCoV antibody [S1MR] (STJ11101211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

SARS-CoV-2

£129.50 - £574.50

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Anti-22KD antibody (STJ11106229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Z.mays

£146.50 - £642.50

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Dot-blot analysis of Biotin-BSA-25-hydroxyvitamin D3 3-hemisuccinic acid using Vitamin D (25-OH-VD) Rabbit monoclonal antibody (STJ11105669) at 1:1000 dilution.

Anti-25-OH-VD antibody [S5669RM] (STJ11105669)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Species independent

£146.50 - £642.50

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Anti-27KD antibody (STJ11106228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Z.mays

£146.50 - £642.50

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STJ72390 (0. 5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-38231 antibody (33-44) (STJ72390)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/FC

Reactivity :

Human/Mouse

£312.50

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STJ72389 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-38231 antibody (425-435) (STJ72389)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/FC

Reactivity :

Human

£312.50

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STJ72674 (1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-38991 antibody (192-204) (STJ72674)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Anti-4-1BBL/CD137L antibody (55-254 aa) (STJ11105922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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STJ70487 staining (0. 25µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-4E-T/EIF4ENIF1 antibody (C-Term) (STJ70487)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Anti-5-HT-TriMethyl-Histone H3-K4 antibody (STJ11106525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Dot-blot analysis of 5-Hydroxymethylcytosine (5-hmC) , 5-Methylcytosine (5mC) and unmodified adenosine using 5-Hydroxymethylcytosine/5-hmC antibody (STJ11103938) at 1:500/1:2000 dilution. NA NA m5C 1- 5 NA CAGTAACTGTGGTCCGGTAACTGACTTGCA3 NA NA NA m5C 2- 5 NA CAGTAACTGTGGTC/i5MedC/GGTAACTGACTTGCA3 NA

Anti-5-Hydroxymethylcytosine/5-hmC antibody (STJ11103938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Dot-blot analysis of all sorts using 5-Methylcytosine (5mC) Rabbit polyclonal antibody (STJ11101496) Line 1：293F cellls transfected with TET2 ProteinLine 2：Another 293F cellls transfected with TET2 ProteinLine 3：293F cellls transfected with GFP-3 ProteinLine 4：DNMT1 knockout (KO) 293F cells

Anti-5-Methyl-Cytosine/5mC antibody (5mC) (STJ11101496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Species independent

£112.50 - £524.50

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The m5C rabbit polyclonal antibody (STJ11103906) are tested in Dot Blot against 5-methylcytidine (m5C) and 5-methylcytosine (5mC).

Anti-5-methylcytidine/m5C antibody (STJ11103906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Species independent

£112.50 - £524.50

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STJ70577 (0. 05ug/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-58K Golgi protein (N-Term) /FTCD antibody (N-Term) (STJ70577)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Pig

£312.50

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Biotinylated STJ70577 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-58K Golgi protein (N-Term) /FTCD, Biotinylated antibody (N-Term) {Biotin} (STJ73168)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Pig

£312.50

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STJ70578 (0. 1µg/ml) staining of Human (A) of Mouse (B) and (0. 3ug/ml) of Pig (C) Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-58KGolgi protein (Internal) /FTCD antibody (Internal) (STJ70578)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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Western blot with Anti-5HT1A Antibody (STJ500000) of rat cortex at 1:250 dilution in buffer on a 14% SDS gel.

Anti-5HT1A antibody (200-250) (STJ500000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-5HT1A antibody (200-250) {Biotin} (STJ500001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-5HT1A antibody (200-250) {FITC} (STJ500002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-5HT1B antibody (160-260) (STJ500003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-5HT1B antibody (160-260) {Biotin} (STJ500004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-5HT1B antibody (160-260) {FITC} (STJ500005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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STJ72883 (2µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-5HT2A Receptor antibody (296-309) (STJ72883)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human

£312.50

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Confocal imaging of paraffin-embedded Mouse brain using 5HT7 Receptor Rabbit monoclonal antibody (STJ11103088, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.

Anti-5HT7 Receptor antibody (1-100) [S8MR] (STJ11103088)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Dot-blot analysis of 5-Methylcytosine (5mC) and unmodified cytosine using 5-Methylcytosine (5mC) Rabbit monoclonal antibody antibody (STJ11104004) at 1:1000 dilution. NA NA 5-Methylcytosine (5mC) : Biotin-5 NA CGATAACCACTAGT (5mC) 3 NA NA NA unmodified cytosine : Biotin-5 NA CGATAACCACTAGTC3 NA

Anti-5mC antibody (5mC) [S4004RM] (STJ11104004)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Species independent

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using 5T4 Rabbit monoclonal antibody (STJ11103034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-5T4 antibody (321-420) [S4MR] (STJ11103034)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Confocal imaging of HeLa cells using 67kDa Laminin Receptor Rabbit monoclonal antibody (STJ11103600, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-67kDa Laminin Receptor antibody (196-295) [S0MR] (STJ11103600)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using A Cyclase V/VI Polyclonal Antibody

Anti-A Cyclase V/VI antibody (931-980 aa) (STJ91397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using ADCY5/6 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-A Cyclase V/VI antibody (931-980 aa) (STJ91398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using A-RAF antibody (STJ11103628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-A-RAF antibody (166-290) [S8MR] (STJ11103628)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-a-Tubulin antibody [TU-16] (STJ16100475)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IP/WB/IHC-P/ICC/ELISA

Reactivity :

Mouse/Rat/Pig/Nicotiana/Human/Dog

£485.50

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Anti-A0A3Q3A7D8 antibody (STJA0010218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Chicken

£48.00 - £252.00

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Anti-A0A3Q3A7D8 antibody (STJA0010219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Chicken

£48.00 - £252.00

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Anti-A0A3Q3A7D8 antibody (STJA0010220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Chicken

£48.00 - £252.00

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Anti-A0A3Q3A7D8 antibody (STJA0010221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Chicken

£48.00 - £252.00

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Western blot analysis of extracts from K562 cells, using A1BG Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-A1BG antibody (100-180 aa) (STJ96422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using A1BG Rabbit polyclonal antibody (STJ113188) at 1:4000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-A1BG antibody (236-495) (STJ113188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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