Anti-4E-T/EIF4ENIF1 antibody (C-Term) (STJ70487)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-4E-T/EIF4ENIF1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recom |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF4ENIF1 |
| Gene ID | 56478 |
| Uniprot ID | 4ET_HUMAN |
| Accession Number | NP_062817.2; NP_001157974.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | AKVISVDELEYRQ |
| Specificity | This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1). |
Additional Info
| Post Translational Modifications | Phosphorylation by MAPK8/JNK1 and or MAPK9/JNK2 in response to oxidative stress promotes P-body assembly. Phosphorylated during meiotic maturation. |
| Function | EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis. Promotes miRNA-mediated translational repression. Required for the formation of P-bodies. Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay. Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism. |
| Protein Name | Eukaryotic Translation Initiation Factor 4e Transporter4e-TEif4e TransporterEukaryotic Translation Initiation Factor 4e Nuclear Import Factor 1 |
| Cellular Localisation | CytoplasmP-BodyNucleusPml BodyNucleus SpecklePredominantly CytoplasmicMainly Localizes To Processing Bodies (P-Bodies)Shuttles Between The Nucleus And The Cytoplasm In A Crm1-Dependent MannerLocalization To Nuclear Foci And Speckles Requires Active Transcription |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4e Transporter antibodyAnti-4e-T antibodyAnti-Eif4e Transporter antibodyAnti-Eukaryotic Translation Initiation Factor 4e Nuclear Import Factor 1 antibodyAnti-EIF4ENIF1 antibody |
Information sourced from Uniprot.org