Primary Antibodies | St John's Laboratory

Western blot analysis of extracts of HeLa cells, using Beta-Catenin antibody (STJ11101750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CTNNB1 antibody (1-100) [S0MR] (STJ11101750)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ Hep G2 cells with 1 Mu g SP1 rabbit monoclonal antibody (STJ11101745) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of SP1 in representative gene loci (TINF2) , as shown in figure.

Anti-SP1 antibody (500-600) [S5MR] (STJ11101745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP rabbit monoclonal antibody (STJ11101660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TARDBP antibody (300-400) [S0MR] (STJ11101660)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (STJ11101574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CCNA2 antibody (11-210) [S4MR] (STJ11101574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AQP4 antibody (256-323) [S2MR] (STJ11101292)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various lysates, using CDK4 rabbit monoclonal antibody (STJ11101279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK4 antibody (204-303) [S9MR] (STJ11101279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£143.50 - £618.50

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Western blot analysis of various lysates using MT-ND5 Rabbit pAb (STJ110434) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-MT-ND5 antibody (500-607) (STJ110434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using NCAM1/ CD56 Rabbit pAb (STJ110222) at 1:500 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 90s.

Anti-NCAM1 antibody (759-858) (STJ110222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from U-251 MG cells using S100A6 Rabbit pAb (STJ11106830) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-S100A6 antibody (1-90 aa) (STJ11106830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SLC6A19 antibody (350-400 aa) (STJ13100522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Marmoset

£322.50

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Anti-VMAT2 antibody (470-517 aa) (STJ13100517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£322.50

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Anti-VGluT3 antibody (STJ13100512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human/Marmoset

£322.50

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Anti-ZO1 antibody (STJ13100476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£322.50

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Anti-VGluT2 antibody (STJ13100472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£322.50

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Anti-Tubulin beta antibody (C-Term) (STJ13100435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human/Xenopus/Zebrafish

£322.50

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Anti-Tau antibody (640-680 aa) (STJ13100432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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Anti-TRPM4 antibody (STJ13100427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£322.50

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Anti-NeuN antibody (STJ13100201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

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Anti-nNOS antibody (STJ13100199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Stargazin antibody (STJ13100088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£322.50

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Anti-Recombinant-Caldesmon/CDM antibody [SM0715] (STJA0010715)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Recombinant-p38 antibody [SR0675] (STJA0010675)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunohistochemistry analysis of p63. Sample: Human cervical carcinoma, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.

Anti-Recombinant-p63 antibody [SM0534] (STJA0010534)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Collagen I antibody [SM0490] (STJA0010490)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunohistochemistry analysis of CD68. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.

Anti-CD68 antibody [SM0477] (STJA0010477)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£153.50 - £228.50

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Immunohistochemistry analysis of CD11c. Sample: Human esophageal cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.

Anti-CD11c antibody [SM0391] (STJA0010391)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£153.50 - £228.50

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Anti-CD31 antibody [SM0378] (STJA0010378)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Rat

£153.50 - £228.50

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Western blot analysis of CK18 at dilution of 1:2000

Anti-Cytokeratin 18 antibody [SM5077] (STJA0005077)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Western blot analysis of Desmin. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-Desmin antibody [SM4942] (STJA0004942)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse

£153.50 - £228.50

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Immunohistochemistry analysis of IBA-1. Sample: mouse brain, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-Iba1 antibody [SM4917] (STJA0004917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IF-P

Reactivity :

Human/Mouse/Rat/Monkey

£153.50 - £228.50

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Western blot analysis of PCNA. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-PCNA antibody [SM4907] (STJA0004907)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ICC

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Flow cytometry:1X10^6 Jurkat cells (negative control, left) and HepG2 cells (right) were surface-stained with CD13/ANPEP Rabbit monoclonal antibody (STJ11104251, 2. 5 Mu g/mL, orange line) or Rabbit IgG isotype control (10 Mu g/mL, blue line) , followed by Alexa Fluor 647 conjugated goat anti-mouse polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-ANPEP antibody (69-967) [S4251RM] (STJ11104251)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Human Kidney tissue was stained with Anti-pVHL (ABT-PVHL) Antibody

Anti-VHL antibody (150-213 aa) [ABT-PVHL] (STJ196864)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£180.00 - £261.00

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Western Blot (WB): Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Claudin 3 STJ196828

Anti-CLDN3 antibody (150-220 aa) [ABT-CLD3] (STJ196828)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£0.00

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Western Blot (WB): Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with STJ196792

Anti-MLH1 antibody (400-500 aa) [ABT-MLH1] (STJ196792)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£0.00

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of H3K18Me1 in representative gene loci (MYT1) , as shown in figure.

Anti-Mono-Methyl-Histone H3-K18 antibody [S1MR] (STJ11103411)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin Rabbit monoclonal antibody (STJ11103153) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Syntaxin antibody (1-100) [S3MR] (STJ11103153)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AIF1/IBA1 antibody (1-100) [S7MR] (STJ11103127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of extracts of various cell lines, using NDUFB8 rabbit monoclonal antibody (STJ11103099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NDUFB8 antibody (1-150) [S9MR] (STJ11103099)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using QKI antibody (STJ11102676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-QK1 antibody (242-341) [S6MR] (STJ11102676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-C-erbB-2 antibody [CB11] (STJ16100742)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,123.50

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Western blot analysis of various lysates using Beta-Actin Rabbit mAb (STJ11100960) at 1:50000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ACTB antibody [S0MR] (STJ11100960)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Zebrafish/Pig

£132.50 - £241.50

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Western blot analysis of various lysates using DDX39A Rabbit pAb (STJ110264) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-DDX39A antibody (1-100) (STJ110264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti FTCD Goat Polyclonal antibody immunohistochemistry human STJ140093

Anti-FTCD antibody (50aa N-Term) (STJ140093)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-P/IHC-F

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £440.50

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Anti TJP1 Goat Polyclonal antibody immunohistochemistry mouse kidney STJ140055

Anti-TJP1 antibody (1580aa C-Term) (STJ140055)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-P/IHC-F

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian/Other

£94.50 - £440.50

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Anti CANX Goat Polyclonal antibody immunohistochemistry human STJ140017

Anti-CANX antibody (550aa C-Term) (STJ140017)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-P/IHC-F

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £686.50

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Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200

Anti-HDGF antibody (141-190 aa) (STJ98794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 293T mouse-kidney lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-EGF antibody (1000-1060 aa) (STJ98714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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