• Western blot analysis of extracts of various cell lines, using NCAM1/CD56 antibody (STJ110222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of SH-SY5Y cells using NCAM1/ CD56 Rabbit polyclonal antibody (STJ110222) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of SH-SY5Y cells, using NCAM1/CD56 antibody (STJ110222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of rat brain cells using NCAM1/ CD56 Rabbit polyclonal antibody (STJ110222) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse brain using NCAM1/ CD56 rabbit polyclonal antibody (STJ110222) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NCAM1/ CD56 in paraffin-embedded rat brain tissue using NCAM1/ CD56 Rabbit polyclonal antibody (STJ110222) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat brain using NCAM1/ CD56 rabbit polyclonal antibody (STJ110222) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NCAM1/ CD56 in paraffin-embedded mouse brain tissue using NCAM1/ CD56 Rabbit polyclonal antibody (STJ110222) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using NCAM1/ CD56 antibody (STJ110222) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from SH-SY5Y cells using NCAM1/ CD56 Rabbit polyclonal antibody (STJ110222) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using NCAM1/ CD56 Rabbit polyclonal antibody (STJ110222) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NCAM1 antibody (759-858) (STJ110222)

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STJ110222

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCAM1/CD56 (759-858) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCAM1
Gene ID: 4684
Uniprot ID: NCAM1_HUMAN
Immunogen Region: 759-858
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1/CD56 (NP_851996.2).
Immunogen Sequence: LCGKAGPGAKGKDMEEGKAA FSKDESKEPIVEVRTEEERT PNHDGGKHTEPNETTPLTEP EKGPVEAKPECQETETKPAP AEVKTVPNDATQTKENESKA
Function This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.
Protein Name Neural Cell Adhesion Molecule 1
N-Cam-1
Ncam-1
Cd Antigen Cd56
Database Links Reactome: R-HSA-3000178
Reactome: R-HSA-375165
Reactome: R-HSA-419037
Reactome: R-HSA-445144
Reactome: R-HSA-5673001
Reactome: R-HSA-877300
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cell Membrane
Isoform 3: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Isoform 4: Cell Membrane
Isoform 5: Secreted
Isoform 6: Secreted
Alternative Antibody Names Anti-Neural Cell Adhesion Molecule 1 antibody
Anti-N-Cam-1 antibody
Anti-Ncam-1 antibody
Anti-Cd Antigen Cd56 antibody
Anti-NCAM1 antibody
Anti-NCAM antibody

Information sourced from Uniprot.org

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