St John's Laboratory Ltd

Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue with MEK2 Mouse mAb diluted at 1:2, 000.

Anti-MAP2K2 antibody [2C3] (STJ97518)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Recombinant-MAP2K2 antibody [RM1V32] (STJA0024511)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse

£300.50 - £457.50

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Western blot analysis of various lysates using FAM129B Rabbit polyclonal antibody (STJ11103204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FAM129B antibody (447-746) (STJ11103204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using COT Polyclonal Antibody

Anti-MAP3K8 antibody (256-305 aa) (STJ92431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using MEK Kinase-2 monoclonal antibody against HEK293 (1) and MAP3K2-hIgGFc transfected HEK293 (2) cell lysate.

Anti-MAP3K2 antibody [4B4] (STJ98236)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-MAP4K5 antibody (300-380 aa) (STJ191700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Recombinant-MAP3K2 antibody [RM2N50] (STJA0026212)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K8 (Phospho-Ser400) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAP3K8-Ser400 antibody (366-415 aa) (STJ91209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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MAP3K8 Blocking Peptide for STJ501677 peptide (STJ504361)

Applications :

Immunodepletion/Immunocompetition

£260.50

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MAP3K8 Positive Control for STJ501675 peptide (STJ504445)

Applications :

WB

£308.50

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Western blot analysis of various cells using Phospho-Cot (T290) Polyclonal Antibody

Anti-Phospho-MAP3K8-Thr290 antibody (256-305 aa) (STJ91027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-MAP3K2 antibody [E6#] (STJA0021584)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR/WB

£198.50 - £1,170.50

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Western blot analysis of extracts of various cell lines, using MEK5 Rabbit monoclonal antibody (STJ11101414) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-MAP2K5 antibody (250-350) [S4MR] (STJ11101414)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of 1) Hela, 2) 3T3, 3) PC12 using MEK-5 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MAP2K5 antibody (STJ197347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-MAP2K5 antibody (STJ196155)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of MCF7 cells using MEK5 Rabbit polyclonal antibody (STJ29033). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP2K5 antibody (1-190) (STJ29033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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MEK5 antibody western blot HeLa lysates STJA0036147

Anti-MEK5 antibody [R05-6E6] (STJA0036147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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SDS-PAGE gel of recombinant human MEK2 protein 3µg under reducing conditions Coomassie blue stain

Human MEK2 protein (Recombinant) (His-Tag) (STJP017633)

Host :

E.coli

Applications :

SDS-PAGE

£285.50 - £2,083.50

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Anti-MAP3K8 antibody (360-467) {Biotin} (STJ501676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Immunohistochemistry of mouse liver with Anti-MAP3K8 primary antibody (STJ501675) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-MAP3K8 antibody (360-467) (STJ501675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-MAP3K8 antibody (360-467) {FITC} (STJ501677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of extracts of 293T cells, using MAP3K8 antibody (STJ118071) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MAP3K8 antibody (1-100) (STJ118071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using MEKK3 Rabbit monoclonal antibody (STJ11101930) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAP3K3 antibody (1-100) [S0MR] (STJ11101930)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using MEKK2 Rabbit monoclonal antibody (STJ11103104) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-MEKK2 antibody (1-100) [S4MR] (STJ11103104)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of MEKK3 in paraffin-embedded human esophageal cancer using MEKK3 Rabbit polyclonal antibody (STJ118498) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAP3K3 antibody (1-220) (STJ118498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western Blot analysis of mouse liver, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MAP3K2-Ser520 antibody (STJA0009920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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MEKK2 antibody western blot C6 3T3 HeLa STJA0011589

Anti-MAP3K2 antibody (11-27 aa) [R06-2K7] (STJA0011589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-MAP3K2 antibody (220-300) (STJA0010100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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MEKK3 antibody western blot HeLa lysates STJA0036175

Anti-MEKK3 antibody [R05-5J8] (STJA0036175)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from HepG2 cells using Phospho-MEK2-T394 Rabbit pAb (STJ22237). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K2-T394 antibody (STJ22237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£126.50 - £618.50

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MEK2 antibody western blot Jurkat cells STJA0036142

Anti-MEK2 antibody [R01-9J3] (STJA0036142)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse

£133.50 - £281.50

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Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit polyclonal antibody (STJ116970) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K2 antibody (1-100) (STJ116970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using [KO Validated] MEK2 Rabbit monoclonal antibody (STJ11101615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAP2K2 antibody (1-100) [S5MR] (STJ11101615)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using IL17RD antibody (STJ112071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IL17RD antibody (150-300) (STJ112071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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MEK2 antibody western blot mouse brain lung STJA0035265

Anti-MEK2 antibody [R05-2G7] (STJA0035265)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-MEK2 antibody [1A2] (STJA0032696)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Monkey/Mouse/Rat

£224.50 - £308.50

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SDS-PAGE gel of recombinant human IER3 protein stained with Coomassie blue

Human IER3 protein (Recombinant) (His-Tag) (STJP018240)

Host :

E.coli

Applications :

SDS-PAGE

£162.50 - £767.50

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Western blot analysis of various lysates using IER3 Rabbit pAb (STJ111301) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-IER3 antibody (50-150) (STJ111301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Human TESC protein (Recombinant) (N-His) (STJP008273)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Immunofluorescence analysis of U2OS cells using MAP4K5 antibody (STJ117333) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP4K5 antibody (300-480) (STJ117333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 293T cells, using Phospho-PERK-T982 Rabbit pAb (STJ11101024) at 1:1000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-EIF2AK3-T982 antibody (STJ11101024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Human MAPK3 protein (Recombinant) (N-His) (STJP004453)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MAPK3 antibody (STJ197394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1：500

Anti-MAPK3 antibody (161-210 aa) (STJ92987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Recombinant-MAPK3 antibody [RM1T31] (STJA0024312)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TESC antibody (20-70 aa) (STJ195252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot phosphorylated MEK5 protein at 49 kDa in rat testes lysate STJA0003686

Anti-Phospho-MAP2K5-Ser311/Thr315 antibody (STJA0003686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Mouse/Xenopus/Zebrafish

£488.50

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Anti-MAPK2 antibody (central Region) (STJA0005412)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£319.50

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