• Immunohistochemistry analysis of MEKK3 in paraffin-embedded human esophageal cancer using MEKK3 Rabbit polyclonal antibody (STJ118498) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MEKK3 in paraffin-embedded human appendix using MEKK3 Rabbit polyclonal antibody (STJ118498) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MEKK3 in paraffin-embedded human lung cancer using MEKK3 Rabbit polyclonal antibody (STJ118498) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MEKK3 Rabbit polyclonal antibody (STJ118498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAP3K3 antibody (1-220) (STJ118498)

SKU:
STJ118498

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP3K3 (1-220) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP3K3
Gene ID: 4215
Uniprot ID: M3K3_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human MEKK3 (NP_976226.1).
Immunogen Sequence: MDEQEALNSIMNDLVALQMN RRHRMPGYETMKNKDTGHSN RQKKHNSSSSALLNSPTVTT SSCAGASEKKKFLSDVRIKF EHNGERRIIAFSRPVKYEDV EHKVTTVFGQPLDLHYMNNE LSILLKNQDDLDKAIDILDR SSSMKSLRILLLSQDRNHNS SSPHSGVSRQVRIKASQSAG DINTIYQPPEPRSRHLSVSS QNPGRSSPPPGYVPERQQHI
Post Translational Modifications Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.
Function Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 3
Mapk/Erk Kinase Kinase 3
Mek Kinase 3
Mekk 3
Database Links Reactome: R-HSA-9020702
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 antibody
Anti-Mapk/Erk Kinase Kinase 3 antibody
Anti-Mek Kinase 3 antibody
Anti-Mekk 3 antibody
Anti-MAP3K3 antibody
Anti-MAPKKK3 antibody
Anti-MEKK3 antibody

Information sourced from Uniprot.org

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