• Western blot analysis of extracts of various cell lines, using MEK5 Rabbit monoclonal antibody (STJ11101414) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of RD cells, using MEK5 Rabbit monoclonal antibody (STJ11101414) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAP2K5 antibody (250-350) [S4MR] (STJ11101414)

SKU:
STJ11101414

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MEK5 (250-350) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K5
Gene ID: 5607
Uniprot ID: MP2K5_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human MEK5 (Q13163).
Immunogen Sequence: DVYRKMPEHVLGRIAVAVVK GLTYLWSLKILHRDVKPSNM LVNTRGQVKLCDFGVSTQLV NSIAKTYVGTNAYMAPERIS GEQYGIHSDVWSLGISFMEL A
Tissue Specificity Expressed in many adult tissues. Abundant in heart and skeletal muscle.
Post Translational Modifications Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. (Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Function Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 5
Map Kinase Kinase 5
Mapkk 5
Mapk/Erk Kinase 5
Mek 5
Database Links Reactome: R-HSA-198765
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 5 antibody
Anti-Map Kinase Kinase 5 antibody
Anti-Mapkk 5 antibody
Anti-Mapk/Erk Kinase 5 antibody
Anti-Mek 5 antibody
Anti-MAP2K5 antibody
Anti-MEK5 antibody
Anti-MKK5 antibody
Anti-PRKMK5 antibody

Information sourced from Uniprot.org

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