• Western blot analysis of lysates from 293 cells treated with UV 15', using COT (Phospho-Thr290) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of HUVEC cells, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Cot (T290) Polyclonal Antibody

Anti-Phospho-MAP3K8-Thr290 antibody (256-305 aa) (STJ91027)

SKU:
STJ91027

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase kinase kinase 8-Thr290 (256-305 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP3K8
Gene ID: 1326
Uniprot ID: M3K8_HUMAN
Immunogen Region: 256-305 aa
Specificity: Phospho-Cot (T290) Polyclonal Antibody detects endogenous levels of Cot protein only when phosphorylated at T290.
Immunogen: The antiserum was produced against synthesized peptide derived from the human COT around the phosphorylation site of Thr290 at the amino acid range 256-305
Post Translational Modifications Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Phosphorylated on Thr-290.the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Phosphorylated on Ser-400 by IKBKB.the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages.
Function Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 8
Cancer Osaka Thyroid Oncogene
Proto-Oncogene C-Cot
Serine/Threonine-Protein Kinase Cot
Tumor Progression Locus 2
Tpl-2
Database Links Reactome: R-HSA-389357
Reactome: R-HSA-5684264
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 antibody
Anti-Cancer Osaka Thyroid Oncogene antibody
Anti-Proto-Oncogene C-Cot antibody
Anti-Serine/Threonine-Protein Kinase Cot antibody
Anti-Tumor Progression Locus 2 antibody
Anti-Tpl-2 antibody
Anti-MAP3K8 antibody
Anti-COT antibody
Anti-ESTF antibody

Information sourced from Uniprot.org

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