St John's Laboratory Ltd

E2F1 Blocking Peptide for STJ500815 peptide (STJ503985)

Applications :

Immunodepletion/Immunocompetition

£260.50

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UBC9 antibody immunocytochemistry HeLa cells STJA0035394

Anti-SUMO Conjugating Enzyme UBC9 antibody [R01-6I6] (STJA0035394)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g [KO Validated] EHMT2 antibody (STJ23499). Western blot was performed from the immunoprecipitate using [KO Validated] EHMT2 antibody (STJ23499) at a dilution of 1:1000.

Anti-EHMT2 antibody (877-1176) (STJ23499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of extracts of A375 cells, using EHMT2 antibody (STJ11103018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EHMT2 antibody (2-300) [S8MR] (STJ11103018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Human MTA1 protein (Recombinant) (N-His) (STJP015907)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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EHMT2 antibody western blot HeLa lysates STJA0037118

Anti-EHMT2 antibody [R05-8H2] (STJA0037118)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various lysates, using p63 rabbit polyclonal antibody (STJ25931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :MCF7Exposure time: 10s.

Anti-TP63 antibody (166-362) (STJ25931)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using PP2A-B56 Gamma/PR61 Gamma/PP2A-B56 Gamma/PR61 Gamma/PPP2R5C antibody (STJ27433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PPP2R5C antibody (1-320) (STJ27433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TP63 antibody (1-100) {Biotin} (STJ502048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-Recombinant-UBE2I/UBC9 antibody [RM2A73] (STJA0025047)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-CUL4A antibody [RM2E82] (STJA0025452)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Human CSNK1D protein (Recombinant) (N-His) (STJP004800)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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p63 antibody western blot A431 lysates STJA0036019

Anti-p63 antibody [R06-1E9] (STJA0036019)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-p63 antibody (STJA0037772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£244.50 - £335.50

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Anti-Phospho-Chk1-Ser317 antibody [PT0744R] (STJA0040059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Human CHEK1 protein (Recombinant) (N-His) (STJP015221)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Chk1 antibody western blot K562 lysates STJA0034168

Anti-Chk1 antibody [R07-4K2] (STJA0034168)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Chk1 S280 antibody western blot 293T Calyculin A STJA0035702

Anti-Phospho-Chk1-Ser280 antibody [R32-4D-1] (STJA0035702)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of 293T cells using Phospho-Chk1 (S317) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser317 antibody (286-335 aa) (STJ90222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using Chk1 monoclonal antibody against A431 (1) , HeLa (2) , NIH/3T3 (3) and K562 (4) cell lysate.

Anti-CHEK1 antibody [2G1D5] (STJ97955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit monoclonal antibody (STJ11104149) at1:2000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-CHEK1 antibody [S4149RM] (STJ11104149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of 293T, using Phospho-Chk1-S280 Rabbit monoclonal antibody (STJ11105894) at 1:2000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-Phospho-CHEK1-S280 antibody [S5894RM] (STJ11105894)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-DDX3X-Thr322 antibody (466-515 aa) (STJ91303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-DDX3X antibody (100-200) (STJ11105653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various cells using DDX3 Polyclonal Antibody

Anti-DDX3X antibody (466-515 aa) (STJ92676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Human DDX3X protein (Recombinant) (N-His) (STJP008111)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S296 Rabbit mAb (STJ11102618) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes and Doxorubicin (0. 5 uM) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CHEK1-S296 antibody [S8MR] (STJ11102618)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-Chk1-S317 Rabbit pAb (STJ22072). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CHEK1-S317 antibody (STJ22072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit pAb (STJ29344) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-CHEK1-S345 antibody (STJ29344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser286 antibody (256-305 aa) (STJ90705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Chk1 antibody [SRM13609] (STJ11108339)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S280 Rabbit pAb (STJ22071) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CHEK1-S280 antibody (STJ22071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of 293T cells using Phospho-Chk1 (S280) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser280 antibody (251-300 aa) (STJ90221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk1 (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser345 antibody (310-359 aa) (STJ90223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293T cells using Phospho-Chk1 (S301) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CHEK1-Ser301 antibody (271-320 aa) (STJ90706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of MCF7 cells using Chk1 Polyclonal Antibody

Anti-CHEK1 antibody (286-335 aa) (STJ92267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Recombinant-CHEK1 antibody [RM1D26] (STJA0022921)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Western blot analysis of extracts of various cell lines, using DDX3X antibody (STJ27604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDX3X antibody (17-33) (STJ27604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of Jurkat cells using DDX3 Polyclonal Antibody diluted at 1：2000

Anti-DDX3 antibody (14-63 aa) (STJ92677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot detection of DDX3 in Hela, 3T3, C6, COS7, K562 and Jurkat cell lysate using DDX3 mouse mAb (1:1000 diluted).Predicted band size: 75KDa.Observed band size: 75KDa.

Anti-DDX3X antibody [6G8-F4-E3] (STJ99237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Rat/Mouse/Monkey

£149.00 - £360.00

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Anti-DDX3 antibody (100-200 aa) [SRM5180] (STJ11107047)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Anti-DDX3 antibody [6G8-F4-E3] (STJA0038225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC/IF

Reactivity :

Human/Rat/Mouse/Monkey

£140.50 - £308.50

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Immunohistochemistry analysis of paraffin-embedded human colon using Cullin 4A Rabbit monoclonal antibody (STJ11102235) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CUL4A antibody (660-759) [S5MR] (STJ11102235)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunofluorescence analysis of U2OS cells using Pumilio 1 antibody (STJ115351) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-pum1 antibody (1-130) (STJ115351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using MTA1 antibody (STJ118525) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MTA1 antibody (600-700) (STJ118525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various lysates using E2F1 Rabbit polyclonal antibody (STJ119131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-E2F1 antibody (301-437) (STJ119131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g Cullin 4A antibody (STJ23293). Western blot was performed from the immunoprecipitate using Cullin 4A antibody (STJ23293) at a dilution of 1:1000.

Anti-CUL4A antibody (1-300) (STJ23293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of brain cells using Casein Kinase I Delta Polyclonal Antibody

Anti-CSNK1D antibody (291-340 aa) (STJ92015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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