• Western blot analysis of DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The lane on the right is blocked with the DDX3/DEAD-box Protein 3 (Phospho-Thr322) peptide.
  • Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-DDX3X-Thr322 antibody (466-515 aa) (STJ91303)

SKU:
STJ91303

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ATP-dependent RNA helicase DDX3X-Thr322 (466-515 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX3X
Gene ID: 1654
Uniprot ID: DDX3X_HUMAN
Immunogen Region: 466-515 aa
Specificity: Phospho-DDX3 (T322) Polyclonal Antibody detects endogenous levels of DDX3 protein only when phosphorylated at T322.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DDX3/DEAD-box Protein 3 around the phosphorylation site of Thr322 at the amino acid range 466-515
Post Translational Modifications Phosphorylated by TBK1.the phosphorylation is required for the synergistic induction of IFNB mediated by TBK1 and DDX3X. Phosphorylated by IKBKE at Ser-102 after ssRNA viral infection.enhances the induction of INFB promoter by IRF3. The cytoplasmic form is highly phosphorylated in the G1/S phase of the cell cycle and much less at G2/M. Phosphorylation by CSNK1E may inhibit RNA-stimulated ATPase activity. Upon stimulation of death receptors, including TNFRSF10B, recruited to receptors and cleaved by caspases. Proteolytic fragments remain associated with the receptors. This cleavage presumably inactivates DDX3X anti-apoptotic function.
Function Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Binds RNA G-quadruplex (rG4s) structures, including those located in the 5'-UTR of NRAS mRNA. Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities (Probable). Involved in transcription regulation. Positively regulates CDKN1A/WAF1/CIP1 transcription in an SP1-dependent manner, hence inhibits cell growth. This function requires its ATPase, but not helicase activity. CDKN1A up-regulation may be cell-type specific. Binds CDH1/E-cadherin promoter and represses its transcription. Potentiates HNF4A-mediated MTTP transcriptional activation.this function requires ATPase, but not helicase activity. Facilitates HNF4A acetylation, possibly catalyzed by CREBBP/EP300, thereby increasing the DNA-binding affinity of HNF4 to its response element. In addition, disrupts the interaction between HNF4 and SHP that forms inactive heterodimers and enhances the formation of active HNF4 homodimers. By promoting HNF4A-induced MTTP expression, may play a role in lipid homeostasis. May positively regulate TP53 transcription. Associates with mRNPs, predominantly with spliced mRNAs carrying an exon junction complex (EJC). Involved in the regulation of translation initiation. Not involved in the general process of translation, but promotes efficient translation of selected complex mRNAs, containing highly structured 5'-untranslated regions (UTR). This function depends on helicase activity. Might facilitate translation by resolving secondary structures of 5'-UTRs during ribosome scanning. Alternatively, may act prior to 43S ribosomal scanning and promote 43S pre-initiation complex entry to mRNAs exhibiting specific RNA motifs, by performing local remodeling of transcript structures located close to the cap moiety. Independently of its ATPase activity, promotes the assembly of functional 80S ribosomes and disassembles from ribosomes prior to the translation elongation process. Positively regulates the translation of cyclin E1/CCNE1 mRNA and consequently promotes G1/S-phase transition during the cell cycle. May activate TP53 translation. Required for endoplasmic reticulum stress-induced ATF4 mRNA translation. Independently of its ATPase/helicase activity, enhances IRES-mediated translation.this activity requires interaction with EIF4E. Independently of its ATPase/helicase activity, has also been shown specifically repress cap-dependent translation, possibly by acting on translation initiation factor EIF4E. Involved in innate immunity, acting as a viral RNA sensor. Binds viral RNAs and promotes the production of type I interferon (IFN-alpha and IFN-beta). Potentiate MAVS/RIGI-mediated induction of IFNB in early stages of infection. Enhances IFNB1 expression via IRF3/IRF7 pathway and participates in NFKB activation in the presence of MAVS and TBK1. Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFNB induction, acts as a scaffolding adapter that links IKBKE and IRF3 and coordinates their activation. Involved in the TLR7/TLR8 signaling pathway leading to type I interferon induction, including IFNA4 production. In this context, acts as an upstream regulator of IRF7 activation by MAP3K14/NIK and CHUK/IKKA. Stimulates CHUK autophosphorylation and activation following physiological activation of the TLR7 and TLR8 pathways, leading to MAP3K14/CHUK-mediated activatory phosphorylation of IRF7. Also stimulates MAP3K14/CHUK-dependent NF-kappa-B signaling. Negatively regulates TNF-induced IL6 and IL8 expression, via the NF-kappa-B pathway. May act by interacting with RELA/p65 and trapping it in the cytoplasm. May also bind IFNB promoter.the function is independent of IRF3. Involved in both stress and inflammatory responses. Independently of its ATPase/helicase activity, required for efficient stress granule assembly through its interaction with EIF4E, hence promotes survival in stressed cells. Independently of its helicase activity, regulates NLRP3 inflammasome assembly through interaction with NLRP3 and hence promotes cell death by pyroptosis during inflammation. This function is independent of helicase activity. Therefore DDX3X availability may be used to interpret stress signals and choose between pro-survival stress granules and pyroptotic NLRP3 inflammasomes and serve as a live-or-die checkpoint in stressed cells. In association with GSK3A/B, negatively regulates extrinsic apoptotic signaling pathway via death domain receptors, including TNFRSF10B, slowing down the rate of CASP3 activation following death receptor stimulation. Cleavage by caspases may inactivate DDX3X and relieve the inhibition. Independently of its ATPase/helicase activity, allosteric activator of CSNK1E. Stimulates CSNK1E-mediated phosphorylation of DVL2, thereby involved in the positive regulation of Wnt/beta-catenin signaling pathway. Also activates CSNK1A1 and CSNK1D in vitro, but it is uncertain if these targets are physiologically relevant. ATPase and casein kinase-activating functions are mutually exclusive. May be involved in mitotic chromosome segregation. (Microbial infection) Facilitates hepatitis C virus (HCV) replication. During infection, HCV core protein inhibits the interaction between MAVS and DDX3X and therefore impairs MAVS-dependent INFB induction and might recruit DDX3X to HCV replication complex. (Microbial infection) Facilitates HIV-1 replication. Acts as a cofactor for XPO1-mediated nuclear export of HIV-1 Rev RNAs. This function is strongly stimulated in the presence of TBK1 and requires DDX3X ATPase activity. (Microbial infection) Facilitates Zika virus (ZIKV) replication. (Microbial infection) Facilitates Dengue virus (DENV) replication. (Microbial infection) Facilitates Venezuelan equine encephalitis virus (VEEV) replication.
Protein Name Atp-Dependent Rna Helicase Ddx3x
Cap-Rf
Dead Box Protein 3 - X-Chromosomal
Dead Box - X Isoform
Dbx
Helicase-Like Protein 2
Hlp2
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Nucleus
Cytoplasm
Stress Granule
Inflammasome
Cell Projection
Lamellipodium
Cytoskeleton
Microtubule Organizing Center
Centrosome
Shuttles Between The Nucleus And The Cytosol
Exported From The Nucleus Partly Through The Xpo1/Crm1 System And Partly Through Nxf1/Tap
Localizes To Nuclear Pores On The Outer Side Of The Nuclear Membrane
In The Cytosol
Partly Colocalizes With Mitochondria
At G0
Predominantly Located In Nucleus
In G1/S Phase
Predominantly Cytoplasmic
During Prophase/Prometaphase
Localizes In Close Proximity To The Condensing Chromosomes
During Telophase
Localizes Around The Newly Synthesized Nuclear Membrane And In The Cytoplasm
Colocalizes With Trpv4 At The Plasma Membrane
When Trpv4 Channel Is Activated
Intracellular Ca(2+) Levels Increase And The Calmodulin/Camkii Pathway Is Activated
Relocalizes To The Nucleus
Wnt3a Stimulation Promotes Ddx3 Recruitment To The Plasma Membrane
At The Leading Edge Of Migrating Fibroblasts
Colocalizes With Caprin1 And Pabpc1
Localizes To Centrosome Throughout The Cell Cycle And Associates With Tp53 At Centrosome During Mitosis
Translocates To The Nucleus In Response To Hpiv-3 Virus-Mediated Infection
Alternative Antibody Names Anti-Atp-Dependent Rna Helicase Ddx3x antibody
Anti-Cap-Rf antibody
Anti-Dead Box Protein 3 - X-Chromosomal antibody
Anti-Dead Box - X Isoform antibody
Anti-Dbx antibody
Anti-Helicase-Like Protein 2 antibody
Anti-Hlp2 antibody
Anti-DDX3X antibody
Anti-DBX antibody
Anti-DDX3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance