St John's Laboratory Ltd

Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g MEK1 antibody (STJ11101700). Western blot was performed from the immunoprecipitate using MEK1 (STJ11101700) at a dilution of 1:1000.

Anti-MAP2K1 antibody (1-100) [S0MR] (STJ11101700)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-pS221 Rabbit pAb (STJ22232) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K1-pS221 antibody (STJ22232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-MEK-1/2-Ser218/222 antibody (189-238 aa) (STJ90327)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser221) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEK-1/2-Ser222/226 antibody (193-242 aa) (STJ90328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K1-Thr286 antibody (252-301 aa) (STJ90854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MEK-1/2 antibody (189-238 aa) (STJ94080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-MAP2K1 antibody (300-350) {Biotin} (STJ501658)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-Recombinant-Phospho-MAP2K1/MEK1-Ser298 antibody [RM2C22] (STJA0025194)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-MAP2K1 antibody [RM2C24] (STJA0025196)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-Recombinant-MEK1/2 antibody [RM1A76] (STJA0026747)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Phospho-MEK1 Ser298 antibody immunocytochemistry HT-1080 STJA0034555

Anti-Phospho-MEK1-Ser298 antibody [R07-1I6] (STJA0034555)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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MEK1 antibody immunocytochemistry HeLa cells STJA0035018

Anti-MEK1 antibody [R01-3C2] (STJA0035018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb (STJ11102603) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-T292 antibody [S3MR] (STJ11102603)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of MEK1 in paraffin-embedded mouse kidney using [KO Validated] MEK1 Rabbit polyclonal antibody (STJ114560) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAP2K1 antibody (1-100) (STJ114560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of 3T3-EGF cells using Phospho-MEK-1 (T386) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K1-Thr386 antibody (344-393 aa) (STJ90585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293T-UV cells using MEK-1 Polyclonal Antibody diluted at 1：500

Anti-MAP2K1 antibody (252-301 aa) (STJ94076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry of mouse brain with Anti-MAP2K1 primary antibody (STJ501657) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-MAP2K1 antibody (300-350) (STJ501657)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Western Blot MEK 1-2 phosphorylation at Ser218-Ser222 in NIH 3T3 cell lysates STJA0003683

Anti-Phospho-MAP2K1/2-Ser218/222 antibody (STJA0003683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Human/Bovine/Canine/Chicken/Non-Human Primates/Rat/Xenopus/Zebrafish

£488.50

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Anti-Recombinant-Phospho-MAP2K1/MEK1-S298 antibody [RM2C20] (STJA0025192)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-MAP2K1/MEK1-T292 antibody [RM2C21] (STJA0025193)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£300.50 - £457.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-S217 Rabbit pAb (STJ114560). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K1-S217 antibody (STJ22230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of U2OS cells using MEK1 Rabbit polyclonal antibody (STJ24463). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP2K1 antibody (1-100) (STJ24463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 293 cells using Phospho-MEK-1 (T292) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MAP2K1-Thr292 antibody (261-310 aa) (STJ90326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HEPG2-UV using p-MEK-1 (S298) antibody. Antibody was diluted at 1:500

Anti-Phospho-MAP2K1-Ser298 antibody (264-313 aa) (STJ90489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-MAP2K1 antibody (300-350) {FITC} (STJ501659)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-MEK1 antibody [1F9] (STJA0032695)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Monkey/Mouse/Rat

£224.50 - £308.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-T292 Rabbit pAb (STJ11103753) at 1:1000 dilution. HeLa cells were treated by Nocodazole (100 ng/ml) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MEK1-T292 antibody (STJ11103753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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MEK1 antibody western blot A431 HeLa lysates STJA0015763

Anti-MAP2K1 antibody (1-40 aa) [R07-8C5] (STJA0015763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat/Cow/Dog

£133.50 - £281.50

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Human MAP2K1 protein (Recombinant) (N-His) (STJP005160)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-MEK1 antibody [SRM13234] (STJ11107964)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Human IER3 protein (Recombinant) (N-GST & C-His) (STJP016309)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (STJ11102602) at 1:1000 dilution. Both HeLa cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-S298 antibody [S2MR] (STJ11102602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (STJ22231) at 1:1000 dilution or MEK1 antibody (STJ114560). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAP2K1-S298 antibody (STJ22231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-MEK1-T286 Rabbit pAb (STJ22233) at 1:2000 dilution or MEK1 antibody (STJ114560). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. PC-12 cells were treated by Nocodazole (1 Mu g/mL) at 37 °C for 16 hours or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-MAP2K1-T286 antibody (STJ22233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Recombinant-MEK1/2 antibody [RM2C25] (STJA0025197)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-MAP2K1/MEK1 antibody [RM1A23] (STJA0026694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat/Cow/Dog

£300.50 - £457.50

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Anti-Recombinant-MAP2K1/MEK1 antibody [RM1B91] (STJA0026861)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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MEK1 antibody western blot A431 cells STJA0037554

Anti-MEK1 antibody (STJA0037554)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Human MAP3K8 protein (Recombinant) (N-His) (STJP015745)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-Recombinant-MAP3K3 antibody [RM2P15] (STJA0026276)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Human MAP4K5 protein (Recombinant) (N-His) (STJP006269)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis of various cells using MEK Kinase-3 Polyclonal Antibody diluted at 1：2000

Anti-MAP3K3 antibody (101-150 aa) (STJ94073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K2-Thr394 antibody (261-310 aa) (STJ90329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using MEK-2 monoclonal antibody against PC-12 (1) , Jurkat (2) , HeLa (3) and NIH/3T3 (4) cell lysate.

Anti-MAP2K2 antibody [7F5] (STJ98237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Western blot detection of MEK1/2 in A549, MCF7 and Hela cell lysates using MEK1/2 mouse mAb (dilution 1:500).Predicted band size:45kDa.Observed band size:45kDa.

Anti-MEK1 antibody [6F10-D7-G6] (STJ99087)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£149.00 - £360.00

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STJ73059 (0. 3µg/ml) staining of Daudi (A) , Jurkat (B) , K562 (C) and Molt4 (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MAP2K2/MEK2 antibody (Internal) (STJ73059)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue with MEK2 Mouse mAb diluted at 1:2, 000.

Anti-MAP2K2 antibody [2C3] (STJ97518)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Recombinant-MAP2K2 antibody [RM1V32] (STJA0024511)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse

£300.50 - £457.50

Choose Options