• Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (STJ22231) at 1:1000 dilution or MAP2K1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g Phospho-MEK1-S298 polyclonal antibody (STJ22231). Western blot was performed from the immunoprecipitate using Phospho-MEK1-S298 polyclonal antibody (STJ22231) at a dilution of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight.
  • Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (STJ22231) at 1:1000 dilution. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by ATP (5 mM) at 30 °C for 1 hour. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit polyclonal antibody (STJ22231) at 1:1000 dilution. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by ATP (5 mM) at 30 °C for 1 hour. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-MAP2K1-S298 polyclonal antibody (STJ22231). Western blot was performed from the immunoprecipitate using Phospho-MAP2K1-S298 polyclonal antibody (STJ22231) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight.
  • Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit polyclonal antibody (STJ22231) at 1:1000 dilution or MEK1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-MAP2K1-S298 antibody (STJ22231)

SKU:
STJ22231

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MAP2K1-S298 is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K1
Gene ID: 5604
Uniprot ID: MP2K1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S298 of human MEK1 (NP_002746.1).
Immunogen Sequence: PLSSY
Tissue Specificity Widely expressed, with extremely low levels in brain.
Post Translational Modifications Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation. (Microbial infection) Acetylation by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 1
Map Kinase Kinase 1
Mapkk 1
Mkk1
Erk Activator Kinase 1
Mapk/Erk Kinase 1
Mek 1
Database Links Reactome: R-HSA-110056
Reactome: R-HSA-170968
Reactome: R-HSA-445144
Reactome: R-HSA-5210891
Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-5684264
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9652169
Reactome: R-HSA-9656223
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole Body
Nucleus
Membrane
Peripheral Membrane Protein
Localizes At Centrosomes During Prometaphase
Midzone During Anaphase And Midbody During Telophase/Cytokinesis
Membrane Localization Is Probably Regulated By Its Interaction With Ksr1
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 antibody
Anti-Map Kinase Kinase 1 antibody
Anti-Mapkk 1 antibody
Anti-Mkk1 antibody
Anti-Erk Activator Kinase 1 antibody
Anti-Mapk/Erk Kinase 1 antibody
Anti-Mek 1 antibody
Anti-MAP2K1 antibody
Anti-MEK1 antibody
Anti-PRKMK1 antibody

Information sourced from Uniprot.org

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