Human MAP3K8 protein (Recombinant) (N-His) (STJP015745)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human MAP3K8-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | MAP3K8 |
| Gene ID | 1326 |
| Uniprot ID | M3K8_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser74-Asn389 |
Additional Info
| Post Translational Modifications | Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Phosphorylated on Thr-290.the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Phosphorylated on Ser-400 by IKBKB.the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages. |
| Function | Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 8Cancer Osaka Thyroid OncogeneProto-Oncogene C-CotSerine/Threonine-Protein Kinase CotTumor Progression Locus 2Tpl-2 |
| Database Links | Reactome: R-HSA-389357Reactome: R-HSA-5684264 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Mitogen-Activated Protein Kinase Kinase Kinase 8 proteinCancer Osaka Thyroid Oncogene proteinProto-Oncogene C-Cot proteinSerine/Threonine-Protein Kinase Cot proteinTumor Progression Locus 2 proteinTpl-2 proteinMAP3K8 proteinCOT proteinESTF protein |
Information sourced from Uniprot.org