Signal Transduction | Research Area | St John's Laboratory

Western blot analysis using GCG monoclonal antibody against HEK293 (1) and GCG-hIgGFc transfected HEK293 (2) cell lysate.

Anti-GCG antibody [2F9] (STJ98103)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of rat liver cells using GCGR Rabbit polyclonal antibody (STJ118008) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-GCGR antibody (50-150) (STJ118008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Glucagon Receptor Polyclonal Antibody

Anti-GCGR antibody (95-144 aa) (STJ93282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200

Anti-GDF2 antibody (371-420 aa) (STJ98776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GDNF antibody (100-180 aa) (STJ192508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-GDNF antibody (109-211 aa) (STJ11106138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using GDNF antibody (STJ116846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GDNF antibody (109-211) (STJ116846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-GDNF antibody [3C1] (STJA0002911)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF/IHC

Reactivity :

Human

£438.50 - £2,502.50

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Anti-GDNF Receptor alpha 1/GFRA1 antibody (361-460 aa) [SRM] (STJ11107301)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using GFAP antibody (STJ11101596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GFAP antibody (1-100) [S6MR] (STJ11101596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of mouse brain, using GFAP rabbit polyclonal antibody (STJ23770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GFAP antibody (1-75) (STJ23770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, GFAP Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-GFAP antibody (11-60 aa) (STJ93259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human brain stained with anti-GFAP antibody using peroxidase-conjugate and DAB chromogen. Note positive staining of astrocytic processes

Anti-GFAP antibody (150-250aa) [ZR356] (STJ180514)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £572.50

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Anti-GFAP antibody (250-300 aa) (STJ13100497)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£331.50

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Anti-GFAP antibody (300-432 aa) [ABT-GFAP] (STJ196765)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GFAP (ABT176) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Neuro-2aPredicted band size: 50kDaObserved band size: 57kDa

Anti-GFAP antibody (300-432 aa) [ABT176] (STJ197157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Human astrocytoma tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT331) Antibody

Anti-GFAP antibody (300-432 aa) [ABT331] (STJ197231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Human astrocytoma tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT470) Antibody

Anti-GFAP antibody (300-432 aa) [ABT470] (STJ190068)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-GFAP antibody (300-432 aa) [ABT484] (STJ197230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Human cerebrum tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT518) Antibody

Anti-GFAP antibody (300-432 aa) [PT1995] (STJ196968)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Fluorescence multiplex immunohistochemical analysis of mouse brain tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit. GFAP mouse monoclonal antibody (STJA0006242 green), S100 mouse monoclonal antibody (STJ197266 cyan), Neurofilament mouse monoclonal antibody (purple), Iba 1 mouse monoclonal antibody (red), CD8 a mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

Anti-GFAP antibody (300-432) [PTR2201/470] (STJA0006242)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-251MG cells using GFAP Rabbit polyclonal antibody (STJ116876) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GFAP antibody (350-432) (STJ116876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-GFAP antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-GFAP antibody (375aa C-Term) (STJ140132)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey

£135.50 - £394.50

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Immunohistochemistry of mouse brain with Anti-GFAP primary antibody (STJ501145) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GFAP antibody (50-100) (STJ501145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-GFAP antibody (50-100) {Biotin} (STJ501146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-GFAP antibody (50-100) {FITC} (STJ501147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of Hela cell. 1, AR Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-GFAP antibody [5C8] (STJ96961)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-GFAP antibody [EP672Y] (STJ16100372)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£630.50 - £1,157.50

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Human brain stained with anti-GFAP antibody using peroxidase-conjugate and DAB chromogen. Note positive staining of astrocytic processes.

Anti-GFAP antibody [GA‐5] (STJ180110)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £572.50

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Western blot analysis of GFAP at dilution of 1:1000

Anti-GFAP antibody [SM0401] (STJA0010401)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-GFAP antibody [SM0407] (STJA0010407)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-GFAP antibody [SM5031] (STJA0005031)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GFOD1 antibody (77-127 aa) (STJ194638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-GFRa-1 antibody (STJA0002877)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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Anti-GFRa-2 antibody (STJA0002878)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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Anti-GFRa-3 antibody (STJA0002879)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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Anti-GFRa-4 antibody (STJA0002885)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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Immunofluorescence analysis of HepG2 cells using GFRA1 Rabbit polyclonal antibody (STJ27326) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GFRA1 antibody (345-420) (STJ27326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GFR alpha-1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-GFRA1 antibody (51-100 aa) (STJ93261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-GFRA2 antibody (320-400 aa) (STJ192348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using GFRA2 antibody (STJ23776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GFRA2 antibody (360-464) (STJ23776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using GFRA3 Rabbit polyclonal antibody (STJ23777) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GFRA3 antibody (32-240) (STJ23777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GFRA3 antibody (STJ192302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using GFRA4 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-GFRA4 antibody (141-190 aa) (STJ93262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GHRH using GHRH Rabbit monoclonal antibody (STJ11105055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GHRH antibody (1-100 aa) [S5055RM] (STJ11105055)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using GHRH antibody (STJ27296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GHRH antibody (37-55) (STJ27296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-GHRH antibody (43-93 aa) (STJ196624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using GHRHR antibody (STJ110719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-GHRHR antibody (1-130) (STJ110719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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