Anti-Cleaved-ADGRE2-Leu517 antibody (517-518) (STJA0005855)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human EMR2 (Cleaved-Leu517)
General Information
| Short Description | Rabbit polyclonal anti-Cleaved-Adhesion G protein-coupled receptor E2-Leu517 (517-518) for use in WB and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:1000-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ADGRE2 |
| Gene ID | 30817 |
| Uniprot ID | AGRE2_HUMAN |
| Immunogen | Synthesized peptide derived from human EMR2 (Cleaved-Leu517) |
| Immunogen Region | 517-518 |
| Specificity | This antibody detects endogenous levels of Human EMR2 (Cleaved-Leu517, protein was cleaved amino acid sequence between 517-518 ) |
Additional Info
| Tissue Specificity | Expression is restricted to myeloid cells. Highest expression was found in peripheral blood leukocytes, followed by spleen and lymph nodes, with intermediate to low levels in thymus, bone marrow, fetal liver, placenta, and lung, and no expression in heart, brain, skeletal muscle, kidney, or pancreas. Expression is also detected in monocyte/macrophage and Jurkat cell lines but not in other cell lines tested. High expression in mast cells. |
| Post Translational Modifications | Autoproteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane beta subunit. |
| Function | Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G-proteins. Is a regulator of mast cell degranulation. |
| Protein Name | Adhesion G Protein-Coupled Receptor E2Egf-Like Module Receptor 2Egf-Like Module-Containing Mucin-Like Hormone Receptor-Like 2Cd Antigen Cd312 |
| Database Links | Reactome: R-HSA-373080 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionRuffle MembraneLocalized At The Leading Edge Of Migrating Cells |
| Alternative Antibody Names | Anti-Adhesion G Protein-Coupled Receptor E2 antibodyAnti-Egf-Like Module Receptor 2 antibodyAnti-Egf-Like Module-Containing Mucin-Like Hormone Receptor-Like 2 antibodyAnti-Cd Antigen Cd312 antibodyAnti-ADGRE2 antibodyAnti-EMR2 antibody |
Information sourced from Uniprot.org