Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (STJ110193) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHD1 antibody (C-Term) (STJ110193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using CHD3 rabbit monoclonal antibody (STJ11102316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHD3 antibody (1-100) [S6MR] (STJ11102316)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using CHD3 antibody (STJ111133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CHD3 antibody (1900-2000) (STJ111133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Mi2-Alpha monoclonal antibody against Y7P, Raji cell lysate.

Anti-CHD3 antibody (C-Term) (STJ98518)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Cow/Dog/Pig

£144.00 - £348.00

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Western blot detection of CHD3 (C-terminus) in Y79 and Raji cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 226KDa.Observed band size: 260KDa.

Anti-CHD3 antibody (C-Term) [4B1-F1-G4] (STJ99070)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/FC

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of CHD3 (C-terminus) in NTERA2, 3T3, C6, K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.

Anti-CHD3 antibody (C-Term) [7F6-G2-E5] (STJ99071)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis using Mi2-Alpha monoclonal antibody against HeLa (1) , K562 (2) , Jurkat (3) , NTERA-2 (4) , HEK293 (5) , Raji (6) cell lysate and mouse brain (7) tissue lysate.

Anti-CHD3 antibody [2G4] (STJ98244)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Immunofluorescence analysis of U20S cells using CHD4 antibody (STJ113182) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CHD4 antibody (1520-1690) (STJ113182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using CHD4 Rabbit monoclonal antibody (STJ11105141) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CHD4 antibody (1520-1700 aa) [S5141RM] (STJ11105141)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of HepG2 cells, using CHD4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CHD4 antibody (571-620 aa) (STJ94119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot detection of CHD4 in K562, HelaNE, HL-60, MOLT-4, Jurkat and 293 cell lysates using CHD4 mouse mAb (1:1000 diluted).Predicted band size:260KDa.Observed band size:260KDa.

Anti-CHD4 antibody [5F1-A11-C9] (STJ99175)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/CoIP

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-CHD8 antibody (STJ190698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using Chk1 Rabbit polyclonal antibody (STJ11104296) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: Q60s.

Anti-CHEK1 antibody (148-307) (STJ11104296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using Chk1 Rabbit polyclonal antibody (STJ29967) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-CHEK1 antibody (148-307) (STJ29967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of K-562 cells, using Chk1 antibody (STJ11104686) at1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CHEK1 antibody (250-350) [S4686RM] (STJ11104686)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of MCF7 cells using Chk1 Polyclonal Antibody

Anti-CHEK1 antibody (286-335 aa) (STJ92267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Chk1 monoclonal antibody against A431 (1) , HeLa (2) , NIH/3T3 (3) and K562 (4) cell lysate.

Anti-CHEK1 antibody [2G1D5] (STJ97955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using CHN1 antibody (STJ29684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CHN1 antibody (1-200) (STJ29684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CHN1 antibody (STJ500525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CHN1 antibody (STJA0008291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CHN1 antibody {Biotin} (STJ500528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CHN1 antibody {FITC} (STJ500529)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot with Anti-CHN2 Antibody (STJ500527) with peptide on at 1:500 dilution in buffer.

Anti-CHN2 antibody (1-100) (STJ500527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CHN2 antibody (1-100) {Biotin} (STJ500532)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CHN2 antibody (1-100) {FITC} (STJ500533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CHN2 antibody (1-200) (STJ111666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry of rat tissue with Anti-CHN2 primary antibody (STJ500526) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CHN2 antibody (150-180) (STJ500526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CHN2 antibody (150-180) {Biotin} (STJ500530)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CHN2 antibody (150-180) {FITC} (STJ500531)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CHN2 antibody (STJA0008963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using CHRDL1 Rabbit polyclonal antibody (STJ117398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHRDL1 antibody (289-458) (STJ117398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis using CHRDL1 monoclonal antibody against rat kidney lysate.

Anti-CHRDL1 antibody (STJ98478)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat

£144.00 - £348.00

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Western blot detection of CHRDL1 in human eye and Hela cell lysates using CHRDL1 mouse mAb (1:1000 diluted).Predicted band size: 57kDa.Observed band size: 52kDa.

Anti-CHRDL1 antibody [3H1-F6-A10] (STJ99125)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of SH-SY5Y cells using CHRM1 Rabbit polyclonal antibody (STJ23130) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-CHRM1 antibody (251-350) (STJ23130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of HepG2 cells using mAChR M1 Polyclonal Antibody

Anti-CHRM1 antibody (295-344 aa) (STJ93976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using CHRM1 Rabbit polyclonal antibody (STJ114206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CHRM1 antibody (400-460) (STJ114206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CHRM1 antibody (6-460) (STJ11101182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (Muscarinic AChR M2 (ACM2) ) rabbit monoclonal antibody (STJ11102277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CHRM2 antibody (100-200) [S7MR] (STJ11102277)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of HeLa cells using mAChR M2 Polyclonal Antibody

Anti-CHRM2 antibody (185-234 aa) (STJ93977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (ACM2) antibody (STJ23131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CHRM2 antibody (208-388) (STJ23131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CHRM3 antibody (270-350 aa) (STJ192646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CHRM3 antibody (STJ116771) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHRM3 antibody (390-475) (STJ116771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of SH-SY5Y, using CHRM3 antibody (STJ29860) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHRM3 antibody (390-590) (STJ29860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using mAChR M4 Polyclonal Antibody

Anti-CHRM4 antibody (236-285 aa) (STJ93978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of NIH/3T3 cells, using CHRM4 antibody (STJ23132). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CHRM4 antibody (261-319) (STJ23132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Mouse spinal cord, using CHRM5 antibody (STJ27320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHRM5 antibody (275-370) (STJ27320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Jurkat cells using mAChR M5 Polyclonal Antibody

Anti-CHRM5 antibody (281-330 aa) (STJ93979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using IKK Alpha antibody (STJ11101770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHUK antibody (1-100) [S0MR] (STJ11101770)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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