Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of various cells using Phospho-Bcl-2 (S70) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-BCL2-Ser70 antibody (41-90 aa) (STJ90191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Monkey

$67.62 - $360.18

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Western blot analysis of 293T-UV cells using Phospho-Bcl-2 (S87) Polyclonal Antibody diluted at 1：500

Anti-Phospho-BCL2-Ser87 antibody (61-110 aa) (STJ90806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Phospho-Bcl-2-T56 Rabbit pAb (STJ22038). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-BCL2-T56 antibody (STJ22038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various cells using Phospho-Bcl-2 (T56) Polyclonal Antibody diluted at 1：500

Anti-Phospho-BCL2-Thr56 antibody (26-75 aa) (STJ90192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using BCL-2 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BCL2-Thr69 antibody (46-95 aa) (STJ90193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-BCL2-Thr74 antibody (STJ91281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from HeLa cells using Phospho-Bcl-XL-S62 Rabbit pAb (STJ22039). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-BCL2L1-S62 antibody (STJ22039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BCL-XL (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BCL2L1-Ser62 antibody (28-77 aa) (STJ90194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BCL-XL (Phospho-Thr115) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BCL2L1-Thr115 antibody (81-130 aa) (STJ91229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of RAT-MUSLE using Phospho-Bcl-x (T47) antibody. Antibody was diluted at 1:500

Anti-Phospho-BCL2L1-Thr47 antibody (13-62 aa) (STJ90195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-BCL2L11-Ser59 antibody (31-80 aa) (STJ91225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-BCL2L11-Ser69 antibody (STJ194702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-BCL2L11-Ser77 antibody (STJ194703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western Blot analysis of 1 Jurkat cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BCL2L11-Thr56/116 antibody (STJA0010071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-BCL9L-Ser915 antibody (STJ196251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Bcr (Y177) Polyclonal Antibody

Anti-Phospho-BCR-Tyr177 antibody (144-193 aa) (STJ90196)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human brain, using Bcr (Phospho-Tyr360) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BCR-Tyr360 antibody (331-380 aa) (STJ91065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$67.62 - $360.18

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Western blot analysis of lysates from K562 cells, using Phospho-BCR-Y177 Rabbit pAb (STJ29372) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-BCR-Y177 antibody (STJ29372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Anti-Phospho-beta Arrestin 1-Ser412 antibody [R05-6F7] (STJA0035053)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-beta Catenin-Ser33/Ser37 antibody [R03-5O3] (STJA0036213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$184.23 - $388.47

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Anti-Phospho-Beta-Catenin-Tyr-142 antibody (STJA0041137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Beta-Catenin-Tyr-333 antibody (STJA0041158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Beta-Catenin-Tyr-489 antibody (STJA0041156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Beta-Catenin-Tyr-654 antibody (STJA0041163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Beta-Catenin-Tyr-86 antibody (STJA0041141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Beta-Tubulin-Ser-172 antibody (STJA0041423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$748.65

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Immunohistochemistry analysis of paraffin-embedded human brain, using Survivin (Phospho-Thr117) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BIRC5-Thr117 antibody (86-135 aa) (STJ91292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HT29 cells treated with EGF 100ug/ml 30', using Survivin (Phospho-Thr34) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BIRC5-Thr34 antibody (11-60 aa) (STJ90479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Raji cells, using Phospho-B-Raf-S445 Rabbit pAb (A0038). Raji cells were treated by PMA/TPA (200nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-BRAF-S445 antibody (STJ22042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.57 - $853.53

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Western blot analysis of various lysates using Phospho-B-Raf-S728 Rabbit pAb (STJ29342). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-BRAF-S728 antibody (STJ29342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Anti-Phospho-BRAF-Ser-446 antibody (STJA0005559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$507.15

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Western blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-BRAF-Ser446 antibody (411-460 aa) (STJ90940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of 3T3 cells using Phospho-Raf-B (S602) Polyclonal Antibody

Anti-Phospho-BRAF-Ser602 antibody (576-625 aa) (STJ90876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (STJ11102615) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-BRAF-T401 antibody [S5MR] (STJ11102615)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$163.53 - $719.67

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Anti-Phospho-BRAF-Thr401 antibody [R05-9B6] (STJA0033920)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using B-RAF (Phospho-Thr599) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRAF-Thr599 antibody (571-620 aa) (STJ91139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using B-Raf (Phospho-Thr753) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BRAF-Thr753 antibody (717-766 aa) (STJ90621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Lane B &C) Western blot with Anti-Phospho-BTK Antibody (STJ502176) on MDAMB281 cells (A), and TPH cells (C) at 1:500 dilution in buffer.

Anti-Phospho-BTK antibody (544-561) (STJ502176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

$686.55

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Anti-Phospho-BTK antibody (544-561) {Biotin} (STJ502177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

$761.07

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Anti-Phospho-BTK antibody (544-561) {FITC} (STJ502178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

$761.07

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Western Blot analysis of mouse heart, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BTK-Ser179 antibody (STJA0010049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-BTK-Ser180 antibody (STJ196255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Btk (Y223) Polyclonal Antibody

Anti-Phospho-BTK-Tyr223 antibody (188-237 aa) (STJ90447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-BTK-Tyr223 antibody [R09-4V4] (STJA0035963)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

$184.23 - $388.47

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Western blot analysis of various cells using Phospho-Btk (Y551) Polyclonal Antibody

Anti-Phospho-BTK-Tyr551 antibody (516-565 aa) (STJ90448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-BTK-Tyr551 antibody [R07-9C1] (STJA0036067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Western blot analysis of lysates from Raji cells, using Phospho-BTK-Y223 Rabbit mAb (STJ11102633) at 1:500 dilution. Raji cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-BTK-Y223 antibody [S3MR] (STJ11102633)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-Phospho-c Myb-Ser11 antibody [R04-2D3] (STJA0034999)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ChIP

Reactivity :

Human

$184.23 - $388.47

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