Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of various lysates using RNF20 Rabbit polyclonal antibody (STJ119439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNF20 antibody (1-260) (STJ119439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-RNF20 antibody (STJA0007068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using RNF31 Rabbit pAb (STJ110526) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-RNF31 antibody (500-700) (STJ110526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of human breast using RNF31 Polyclonal Antibody (STJ116074) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RNF31 antibody (500-700) (STJ116074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Anti-RNF31 antibody (833-883) (STJA0006389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Anti-RNF41 antibody (1-317 aa) (STJ11107493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RNF41 antibody (STJ190789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using ROCK1 Rabbit monoclonal antibody (STJ112994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ROCK1 antibody (1100-1200) [S4MR] (STJ112994)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of ROCK1 in C6, 3T3, Hela lysates using ROCK1 antibody.

Anti-ROCK1 antibody (1344-1354 aa) [R09-3D3] (STJA0011909)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£264.50

Choose Options

Immunofluorescence analysis of Hela cell. 1, Rock-1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-ROCK1 antibody (262-311 aa) (STJ95527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g ROCK1 antibody (STJ11103795). Western blot was performed from the immunoprecipitate using ROCK1 antibody (STJ11103795) at a dilution of 1:1000.

Anti-ROCK1 antibody (400-660) (STJ11103795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse spleen using ROCK1 antibody (STJ29833) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ROCK1 antibody (400-660) (STJ29833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

Choose Options

Western blot with Anti-ROCK1 Antibody (STJ502819) on rat: 1) Ovary, 2) Heart, 3) Kidney, 4) Brain, 5) Liver, 6) Tongue, 7) Spinal Cord, 8) Cerebellum, 9) Skeletal muscle. Antibody at 1:250 dilution in buffer.

Anti-ROCK1 antibody (850-900) (STJ502819)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£427.50

Choose Options

Anti-ROCK1 antibody (850-900) {Biotin} (STJ502820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£528.50

Choose Options

Anti-ROCK1 antibody (850-900) {FITC} (STJ502821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£528.50

Choose Options

Western blot analysis of lysates from Human lung using ROCK2 Rabbit polyclonal antibody (STJ11104494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ROCK2 antibody (1109-1388) (STJ11104494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Anti-ROCK2 antibody (1109-1388) (STJ27665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of ROCK2 in paraffin-embedded mouse intestin tissue using ROCK2 Rabbit monoclonal antibody (STJ11101818) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-ROCK2 antibody (1289-1388) [S8MR] (STJ11101818)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot with Anti-ROCK2 Antibody (STJ502822) on T47D cells. Antibody at 1:500 dilution in buffer.

Anti-ROCK2 antibody (1300-1388) (STJ502822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

Choose Options

Anti-ROCK2 antibody (1300-1388) {Biotin} (STJ502823)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-ROCK2 antibody (1300-1388) {FITC} (STJ502824)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Western blot analysis of various cells using Rock-2 Polyclonal Antibody diluted at 1：1000

Anti-ROCK2 antibody (691-740 aa) (STJ95528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ROPN1 antibody (85-135 aa) (STJ195586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from Rat uterus, using ROR1 Rabbit polyclonal antibody (STJ25381) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ROR1 antibody (24-937) (STJ25381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

Choose Options

Western blot analysis using ROR1 monoclonal antibody against extracellular domain of human ROR1 (aa30-423).

Anti-ROR1 antibody (30-406 aa) [2H6] (STJ98365)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Anti-ROR1 antibody (STJA0010258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using ROR2 Rabbit monoclonal antibody (STJ11102262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ROR2 antibody (1-100) [S2MR] (STJ11102262)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £574.50

Choose Options

Western blot analysis of Mouse embryo, using ROR2 antibody (STJ27587) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ROR2 antibody (100-200) (STJ27587)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from rat small intestine, using ROR2 Rabbit polyclonal antibody (STJ119459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ROR2 antibody (300-400) (STJ119459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

Choose Options

Anti-ROR2 antibody (450-530 aa) (STJ95530)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunohistochemistry of baboon Bladder with Anti-ROR2 primary antibody (STJ502828) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ROR2 antibody (900-943) (STJ502828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

Choose Options

Anti-ROR2 antibody (900-943) {Biotin} (STJ502829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

Choose Options

Anti-ROR2 antibody (900-943) {FITC} (STJ502830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Western blot analysis of various lysates using RPB3/POLR2C Rabbit mAb (STJ11103678) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-RPB3/POLR2C antibody (1-100) [S8MR] (STJ11103678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Anti-RPGRIP1L antibody (1-300) (STJ11100171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-RPGRIP1L antibody (STJA0006793)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using RPS27 antibody (STJ28812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPS27 antibody (1-84) (STJ28812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human lung cancer (left) and human brain (right) tissues with DAB staining using Ribosomal Protein S27 monoclonal antibody.

Anti-RPS27 antibody [4A12] (STJ98360)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Confocal immunofluorescence analysis of Hela cells (A) , BCBL-1 cells (B) and L1210 cells (C) using Ribosomal Protein S27 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Anti-RPS27 antibody [7E3] (STJ98361)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse kidney using RPS27A antibody (STJ116826) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-RPS27A antibody (1-156) (STJ116826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of various cell lines, using RPS27A antibody (STJ25406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPS27A antibody (1-156) (STJ25406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-RPS27A antibody (STJA0007619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of NIH/3T3 cells using S6 Ribosomal Protein (RPS6) Rabbit polyclonal antibody (STJ27852) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RPS6 antibody (1-130) (STJ27852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates, using RPS6 Mouse mAb (STJ11107006) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 20s.

Anti-RPS6 antibody (1-249 aa) [SMM] (STJ11107006)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Anti-RPS6 antibody at 1:500 dilution; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RPS6 antibody (190aa C-Term) (STJ140049)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

Choose Options

Western blot analysis of various cells using Ribosomal Protein S6 Polyclonal Antibody diluted at 1：2000

Anti-RPS6 antibody (191-240 aa) (STJ95504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using p90Rsk/RSK1/RPS6KA1 Rabbit monoclonal antibody (STJ11102133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RPS6KA1 antibody (1-100) [S3MR] (STJ11102133)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

Choose Options

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-RPS6KA1 antibody (539-588 aa) (STJ95545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options