• Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1-T246 antibody (STJ117891) at 1:2000 dilution. HeLa cells were treated by Insulin (0. 01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of HeLa, using Phospho-AKT1S1/PRAS40-T246 Rabbit polyclonal antibody (STJ117891) at 1:400 dilution. HeLa cells were treated by Insulin (100 nM) at 37 °C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-Phospho-AKT1S1-T246 antibody (STJ117891)

SKU:
STJ117891

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-AKT1S1-T246 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKT1S1
Gene ID: 84335
Uniprot ID: AKTS1_HUMAN
Immunogen: A synthetic phosphorylated peptide around T246 of human AKT1S1/PRAS40 (NP_001092102.1).
Immunogen Sequence: LNTSD
Tissue Specificity Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
Post Translational Modifications Phosphorylated by AKT1.phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity. Phosphorylated by MTOR following mTORC1 activation, inhibiting AKT1S1 inhibitor activity: phosphorylation by MTOR probably serves as a feedback loop that relieves inhibition from AKT1S1 in response to mTORC1 inactivation. Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1.
Function Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site. In response to insulin and nutrients, AKT1S1 dissociates from mTORC1. Its activity is dependent on its phosphorylation state and binding to 14-3-3. May also play a role in nerve growth factor-mediated neuroprotection.
Protein Name Proline-Rich Akt1 Substrate 1
40 Kda Proline-Rich Akt Substrate
Database Links Reactome: R-HSA-165159
Reactome: R-HSA-166208
Reactome: R-HSA-198323
Reactome: R-HSA-3371571
Reactome: R-HSA-5674400
Cellular Localisation Cytoplasm
Cytosol
Found In The Cytosolic Fraction Of The Brain
Alternative Antibody Names Anti-Proline-Rich Akt1 Substrate 1 antibody
Anti-40 Kda Proline-Rich Akt Substrate antibody
Anti-AKT1S1 antibody
Anti-PRAS40 antibody

Information sourced from Uniprot.org

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