• Immunohistochemistry analysis of paraffin-embedded human brain, using ALK (Phospho-Tyr1096) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ALK-Tyr1096 antibody (1062-1111 aa) (STJ91196)

SKU:
STJ91196

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ALK tyrosine kinase receptor-Tyr1096 (1062-1111 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALK
Gene ID: 238
Uniprot ID: ALK_HUMAN
Immunogen Region: 1062-1111 aa
Specificity: Phospho-ALK (Y1096) Polyclonal Antibody detects endogenous levels of ALK protein only when phosphorylated at Y1096.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ALK around the phosphorylation site of Tyr1096 at the amino acid range 1062-1111
Post Translational Modifications Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association. N-glycosylated.
Function Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Also acts as a key thinness protein involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis. Following activation by ALKAL2 ligand at the cell surface, transduces an extracellular signal into an intracellular response. In contrast, ALKAL1 is not a potent physiological ligand for ALK. Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. ALK activation may also be regulated by pleiotrophin (PTN) and midkine (MDK). PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
Protein Name Alk Tyrosine Kinase Receptor
Anaplastic Lymphoma Kinase
Cd Antigen Cd246
Database Links Reactome: R-HSA-201556
Reactome: R-HSA-9700645
Reactome: R-HSA-9717264
Reactome: R-HSA-9717301
Reactome: R-HSA-9717316
Reactome: R-HSA-9717319
Reactome: R-HSA-9717323
Reactome: R-HSA-9717326
Reactome: R-HSA-9717329
Reactome: R-HSA-9725370
Reactome: R-HSA-9725371
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Attachment Is Essential For Promotion Of Neuron-Like Differentiation And Cell Proliferation Arrest Through Specific Activation Of The Map Kinase Pathway
Alternative Antibody Names Anti-Alk Tyrosine Kinase Receptor antibody
Anti-Anaplastic Lymphoma Kinase antibody
Anti-Cd Antigen Cd246 antibody
Anti-ALK antibody

Information sourced from Uniprot.org

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