• Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30', using APC (Phospho-Ser2054) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using APC (Phospho-Ser2054) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-APC-Ser2054 antibody (2020-2069 aa) (STJ90884)

SKU:
STJ90884

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Adenomatous polyposis coli protein-Ser2054 (2020-2069 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APC
Gene ID: 324
Uniprot ID: APC_HUMAN
Immunogen Region: 2020-2069 aa
Specificity: Phospho-APC (S2054) Polyclonal Antibody detects endogenous levels of APC protein only when phosphorylated at S2054.
Immunogen: The antiserum was produced against synthesized peptide derived from the human APC around the phosphorylation site of Ser2054 at the amino acid range 2020-2069
Post Translational Modifications Phosphorylated.phosphorylation enhances the F-actin bundling activity. Phosphorylated by GSK3B. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
Function Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Associates with both microtubules and actin filaments, components of the cytoskeleton. Plays a role in mediating the organization of F-actin into ordered bundles. Functions downstream of Rho GTPases and DIAPH1 to selectively stabilize microtubules. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
Protein Name Adenomatous Polyposis Coli Protein
Protein Apc
Deleted In Polyposis 2.5
Database Links Reactome: R-HSA-111465
Reactome: R-HSA-195253
Reactome: R-HSA-196299
Reactome: R-HSA-3769402
Reactome: R-HSA-4641262
Reactome: R-HSA-5339716
Reactome: R-HSA-5358747
Reactome: R-HSA-5358749
Reactome: R-HSA-5358751
Reactome: R-HSA-5358752
Reactome: R-HSA-5467333
Reactome: R-HSA-5467337
Reactome: R-HSA-5467340
Reactome: R-HSA-5467348
Reactome: R-HSA-5689896
Cellular Localisation Cell Junction
Adherens Junction
Cytoplasm
Cytoskeleton
Cell Projection
Lamellipodium
Ruffle Membrane
Cell Membrane
Associated With The Microtubule Network At The Growing Distal Tip Of Microtubules
Mapre1 May Be Required For Targeting To The Growing Microtubule Plus Ends
Accumulates In The Lamellipodium And Ruffle Membrane In Response To Hepatocyte Growth Factor (Hgf) Treatment
The Memo1-Rhoa-Diaph1 Signaling Pathway Controls Localization Of The Phosphorylated Form To The Cell Membrane
Alternative Antibody Names Anti-Adenomatous Polyposis Coli Protein antibody
Anti-Protein Apc antibody
Anti-Deleted In Polyposis 2.5 antibody
Anti-APC antibody
Anti-DP2.5 antibody

Information sourced from Uniprot.org

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