Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of L929 cells using PDGF-D Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PDGFD antibody (311-360 aa) (STJ96664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-PDGFD antibody [SAA0451] (STJA0027628)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-PDGFD antibody [SAA0451] {APC} (STJA0029743)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-PDGFD antibody [SAA0451] {FITC} (STJA0028777)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-PDGFD antibody [SAA0451] {PerCP} (STJA0030709)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-PDGFD antibody [SAA0451] {PE} (STJA0031675)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-PDGFR alpha antibody (24-524 aa) [SRM5362] (STJ11107272)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£126.50 - £554.50

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PDGFR alpha antibody immunohistochemistry rat skin tissue STJA0032929

Anti-PDGFR alpha antibody [7H6-7C1-10A5] (STJA0032929)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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PDGFR alpha antibody western blot SH-SY5Y STJA0036314

Anti-PDGFR alpha antibody [R05-4A8] (STJA0036314)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-PDGFR Alpha/CD140a antibody {PE} (STJ11108991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human/Mouse

£175.50 - £507.50

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Immunofluorescence analysis of mouse-spleen tissue. 1, PDGFR Alpha Mouse monoclonal antibody (7A3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PDGFRA antibody (1010-1090 aa) [7A3] (STJ97793)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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PDGFR alpha antibody western blot C6 3T3 cells STJA0011760

Anti-PDGFRA antibody (1074-1089 aa) [R06-6D3] (STJA0011760)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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PDGFR alpha antibody western blot 3T3 lysates STJA0011761

Anti-PDGFRA antibody (24-528 aa) [R06-7D7] (STJA0011761)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-PDGFRA antibody (300-400) [PTR1303] (STJA0006268)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of SH-SY5Y cells using PDGFR-Alpha Polyclonal Antibody

Anti-PDGFRA antibody (731-780 aa) (STJ95000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U2OS using PDGFR antibody (STJ24937) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDGFRA antibody (896-1089) (STJ24937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PDGFRa antibody [SRM14918] (STJ11109648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Confocal imaging of NIH/3T3 cells using PDGFRB Rabbit monoclonal antibody (STJ11101680, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PDGFRB antibody (1000-1106) [S0MR] (STJ11101680)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of PDGFR beta in SH-SY5Y, C6 lysates using PDGFR beta antibody.

Anti-PDGFRB antibody (1094-1106 aa) [R02-5B2] (STJA0010882)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£140.50 - £308.50

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Immunofluorescence analysis of NIH/3T3 cells using PDGFRB Rabbit polyclonal antibody (STJ24938) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PDGFRB antibody (241-259) (STJ24938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 1) Hela, 2) K562, 3) KB cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-PDGFRB antibody (718-767 aa) (STJ95004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH-3T3 cells using PDGFRB Rabbit polyclonal antibody (STJ11102763) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PDGFRB antibody (900-1000) (STJ11102763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of COS7 cells, using PDGFR beta Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PDGFRB antibody (991-1040 aa) (STJ95003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using PDGFR-Beta monoclonal antibody against NIH/3T3 cell lysate (1).

Anti-PDGFRB antibody [1A2] (STJ98314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis of extracts of various cell lines, using PDHA1 antibody (STJ11104699) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PDHA1 antibody (248-348) [S4699RM] (STJ11104699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of PDHA1 in paraffin-embedded human thyroid cancer using PDHA1 Rabbit polyclonal antibody (STJ24939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PDHA1 antibody (30-390) (STJ24939)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using PDHA1 Polyclonal Antibody diluted at 1：2000

Anti-PDHA1 antibody (314-363 aa) (STJ95006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Confocal imaging of C2C12 cells using PDHA1 Rabbit monoclonal antibody (STJ11101429, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PDHA1 antibody (50-150) [S9MR] (STJ11101429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis using PDH-E1 Alpha monoclonal antibody against A549, HeLa cell lysate.

Anti-PDHA1 antibody (C-Term) (STJ98528)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Dog/Pig

£149.00 - £360.00

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Anti-PDHA1 antibody (STJA0010259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293, 1299, mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa.

Anti-PDHA1 antibody [3H2-F8-B5] (STJ99056)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis of various lysates using PDHA2 Rabbit pAb (STJ111984) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-PDHA2 antibody (119-388) (STJ111984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDHA2 antibody (310-390 aa) (STJ191234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using PDHB antibody (STJ29023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDHB antibody (1-300) (STJ29023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDHB antibody (160-240 aa) (STJ191235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of PDHB in paraffin-embedded rat kidney tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-PDHB antibody (260-359) [S3MR] (STJ11102123)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g PDHX antibody (STJ28509). Western blot was performed from the immunoprecipitate using PDHX antibody (STJ28509) at a dilution of 1:1000.

Anti-PDHX antibody (1-300) (STJ28509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDHX antibody (24-74 aa) (STJ195078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot of human PDHX antibody STJA0041867 in A431 HepG2 293T and LnCap cell lysates

Anti-PDHX antibody (54-501aa) [AT1E11] (STJA0041867)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

£219.50 - £332.50

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Western blot analysis of various lysates using PDK1/PDHK1 Rabbit monoclonal antibody (STJ11105443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PDK1 antibody (1-100) [S5443RM] (STJ11105443)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunofluorescence analysis of PC-12 cells using PDK1/PDHK1 Rabbit polyclonal antibody (STJ24945) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDK1 antibody (337-436) (STJ24945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using PDK1/PDHK1 antibody (STJ11104825) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDK1 antibody (337-436) [S4825RM] (STJ11104825)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various lysates, using PDK1/PDHK1 Rabbit mAb (STJ11102396) at 1:20000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PDK1 antibody (337-436) [S6MR] (STJ11102396)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various cells using PDK1 Polyclonal Antibody

Anti-PDK1 antibody (80-160 aa) (STJ95009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PDK1 antibody (C-Term) [M146] (STJA0005520)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot mouse brain and human A431 cells probed with anti-PDK1 antibody STJA0041312

Anti-PDK1 antibody (C-Term) [M146] (STJA0041312)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£474.50

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Anti-PDK1 antibody (N-Term) (STJA0005519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot A431 cells anti-PDK1 antibody reactivity with pervanadate treatment STJA0041332

Anti-PDK1 antibody (N-Term) (STJA0041332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£474.50

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