Anti-PDHA1 antibody (30-390) (STJ24939)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PDHA1 (30-390) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PDHA1 |
| Gene ID | 5160 |
| Uniprot ID | ODPA_HUMAN |
| Immunogen Region | 30-390 |
| Immunogen Sequence | FANDATFEIKKCDLHRLEEG PPVTTVLTREDGLKYYRMMQ TVRRMELKADQLYKQKIIRG FCHLCDGQEACCVGLEAGIN PTDHLITAYRAHGFTFTRGL SVREILAELTGRKGGCAKGK GGSMHMYAKNFYGGNGIVGA QVPLGAGIALACKYNGKDEV CLTLYGDGAANQGQIFEAYN MAALWKLPCIFICENNRYGM GTSVERAAASTDYYKRGDFI PGLRVDGMDILCVREATRF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - MitochondrialPdhe1-A Type I |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9837999Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibodyAnti-Pdhe1-A Type I antibodyAnti-PDHA1 antibodyAnti-PHE1A antibody |
Information sourced from Uniprot.org