Anti-PDHX antibody (1-300) (STJ28509)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PDHX (1-300) antibody for use in WB, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PDHX |
| Gene ID | 8050 |
| Uniprot ID | ODPX_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MAASWRLGCDPRLLRYLVGF PGRRSVGLVKGALGWSVSRG ANWRWFHSTQWLRGDPIKIL MPSLSPTMEEGNIVKWLKKE GEAVSAGDALCEIETDKAVV TLDASDDGILAKIVVEEGSK NIRLGSLIGLIVEEGEDWKH VEIPKDVGPPPPVSKPSEPR PSPEPQISIPVKKEHIPGTL RFRLSPAARNILEKHSLDAS QGTATGPRGIFTKEDALKLV QLKQTGKITESRPTPAPTA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PDHX (NP_003468.2). |
Additional Info
| Post Translational Modifications | Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity. |
| Function | Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. |
| Protein Name | Pyruvate Dehydrogenase Protein X Component - MitochondrialDihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase ComplexE3-Binding ProteinE3bpLipoyl-Containing Pyruvate Dehydrogenase Complex Component XProx |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase Protein X Component - Mitochondrial antibodyAnti-Dihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase Complex antibodyAnti-E3-Binding Protein antibodyAnti-E3bp antibodyAnti-Lipoyl-Containing Pyruvate Dehydrogenase Complex Component X antibodyAnti-Prox antibodyAnti-PDHX antibodyAnti-PDX1 antibody |
Information sourced from Uniprot.org