Anti-PDHA1 antibody (C-Term) (STJ98528)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
ImmunogenPurified recombinant human PDH-E1 Alpha (C-terminus) protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (C-Term) for use in WB and IF in Human, Mouse, Rat, Cow, Dog and Pig samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat/Cow/Dog/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity purification |
| Dilution Range | WB 1:1000-1:2000IF 1:100-1:500 |
| Formulation | Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PDHA1 |
| Gene ID | 5160 |
| Uniprot ID | ODPA_HUMAN |
| Immunogen | Purified recombinant human PDH-E1 Alpha (C-terminus) protein fragments expressed in E.coli. |
| Immunogen Region | C-Term |
| Specificity | PDH-E1 Alpha Monoclonal Antibody detects endogenous levels of PDH-E1 Alpha protein. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - MitochondrialPdhe1-A Type I |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9837999Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibodyAnti-Pdhe1-A Type I antibodyAnti-PDHA1 antibodyAnti-PHE1A antibody |
Information sourced from Uniprot.org