Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using HB-EGF antibody (STJ11101381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HBEGF antibody (50-150) [S1MR] (STJ11101381)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-HBEGF antibody [PT1429R] (STJA0040326)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-HBEGF antibody [R05-9X9] (STJA0036838)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-HBEGF antibody [R08-9C2] (STJA0034398)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-HBEGF antibody [SAA0453] (STJA0027630)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£231.50 - £286.50

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Anti-HBEGF antibody [SAA0453] {APC} (STJA0029661)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£282.50 - £352.50

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Anti-HBEGF antibody [SAA0453] {FITC} (STJA0028695)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£231.50 - £284.50

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Anti-HBEGF antibody [SAA0453] {PerCP} (STJA0030627)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£265.50 - £330.50

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Anti-HBEGF antibody [SAA0453] {PE} (STJA0031593)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£282.50 - £352.50

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Anti-HBEGF nanobody [SAA1356] (STJN000296)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of lysates from Mouse brain, using HCAR1 Rabbit polyclonal antibody (STJ11103269) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HCAR1 antibody (250-346) (STJ11103269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HCAR1 antibody (STJ192658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from PC-12 cells, using GPR109A/HM74A/HCAR2 Rabbit monoclonal antibody (STJ11104625) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-HCAR2 antibody (100-200) [S4625RM] (STJ11104625)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from BxPC-3 cells using GPR109A/HM74A/HCAR2 rabbit polyclonal antibody (STJ118060) at 1:400 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:180s.

Anti-HCAR2 antibody (165-182) (STJ118060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HCAR2 antibody (260-340 aa) (STJ192810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of HCAR3 in paraffin-embedded Human spleen using HCAR3 Rabbit polyclonal antibody (STJ11100354) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HCAR3 antibody (300-392) (STJ11100354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-hCG antibody [SM5034] (STJA0005034)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HCRT antibody (21-70 aa) (STJ192585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using HCRT Rabbit pAb (STJ111863) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-HCRT antibody (34-66) (STJ111863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of paraffin-embedded mouse brain using HCRTR1 Rabbit polyclonal antibody (STJ116940) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HCRTR1 antibody (150-250) (STJ116940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from RAW264.7 cells, using HCRTR1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-HCRTR1 antibody (231-280 aa) (STJ94836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of MCF7 cells, using HCRTR1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HCRTR1 antibody (298-347 aa) (STJ94837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HCRTR1/OX1R antibody [SAA1954] (STJA0020382)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Anti-HCRTR2 antibody (280-360 aa) (STJ192775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using HCRTR2 antibody (STJ23927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HCRTR2 antibody (380-444) (STJ23927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HDAC1 (PTR1326) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 55kDaObserved band size: 62kDa

Anti-HDAC1 antibody (300-482) [PTR1326] (STJA0006279)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunoprecipitation analysis of 300ug extracts of 293T cells using 0. 5ug HDAC1 antibody (STJ11101705). Western blot was performed from the immunoprecipitate using HDAC1 antibody (STJ11101705) at a dilition of 1:1000.

Anti-HDAC1 antibody (350-450) [S5MR] (STJ11101705)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various cells using HDAC1 Polyclonal Antibody diluted at 1：500

Anti-HDAC1 antibody (387-436 aa) (STJ93477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of HT-29 cells, using 3 Mu g [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928). Western blot was performed from the immunoprecipitate using HDAC1 antibody (STJ23928) at a dilution of 1:1000.

Anti-HDAC1 antibody (393-482) (STJ23928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Histone deacetylase 1 Polyclonal Antibody diluted at 1：1000

Anti-HDAC1 antibody (433-482 aa) (STJ93510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HDAC1 antibody (Internal Region) (STJ13101199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-HDAC1 antibody [3F5-2E8-1F7] (STJA0032893)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Anti-HDAC1 antibody [PT0390R] (STJA0039585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-HDAC1 antibody [R07-8M-9] (STJA0033696)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£214.50 - £308.50

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Anti-HDAC1 antibody [R09-7G9] (STJA0034400)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-HDAC1 antibody [SQab1867] (STJ16102880)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/ICC/IF/IHC-P/WB

Reactivity :

Human/Mouse/Rat

£708.50

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Anti-HDAC1 antibody [SRM14909] (STJ11109639)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/IF-P/IHC-P/ChIP/ELISA

Reactivity :

Human/Mouse

£0.00

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Western blot analysis of various cells using Histone deacetylase 10 Polyclonal Antibody diluted at 1：1000

Anti-HDAC10 antibody (10-59 aa) (STJ93511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-HDAC10 antibody (STJA0005751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£488.50

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Anti-HDAC10 antibody (STJA0037565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-HDAC10 antibody [R06-6K7] (STJA0036154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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Western blot analysis of lysates from Mouse brain using HDAC11 Rabbit mAb (STJ11107108) at 1:1000 dilution incubated at room temperature for 1. 5 hours. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.

Anti-HDAC11 antibody (1-347 aa) [SRM5226] (STJ11107108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HuvEc cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HDAC11 antibody (170-220 aa) (STJ194849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using HDAC11 antibody (STJ27893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HDAC11 antibody (270-347) (STJ27893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HDAC11 antibody [R05-4K8] (STJA0035745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-HDAC11 antibody [R07-7B8] (STJA0034401)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-HDAC11 antibody [SAA1877] (STJA0020305)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g HDAC2 antibody (STJ11101736). Western blot was performed from the immunoprecipitate using HDAC2 antibody (STJ11101736) at a dilution of 1:1000.

Anti-HDAC2 antibody (1-100) [S6MR] (STJ11101736)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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