• Western blot analysis of extracts of various cell lines, using HB-EGF antibody (STJ11101381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HBEGF antibody (50-150) [S1MR] (STJ11101381)

SKU:
STJ11101381

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HB EGF (50-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HBEGF
Gene ID: 1839
Uniprot ID: HBEGF_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HB-EGF (Q99075).
Immunogen Sequence: LLPLGGGRDRKVRDLQEADL DLLRVTLSSKPQALATPNKE EHGKRKKKGKGLGKKRDPCL RKYKDFCIHGECKYVKELRA PSCICHPGYHGERCHGLSLP V
Post Translational Modifications Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active. O-glycosylated with core 1 or possibly core 8 glycans. Thr-47 is a minor glycosylation site compared to Thr-44.
Function Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Protein Name Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth Factor
Hb-Egf
Hbegf
Diphtheria Toxin Receptor
Dt-R
Database Links Reactome: R-HSA-1227986
Reactome: R-HSA-1236394
Reactome: R-HSA-1250196
Reactome: R-HSA-1250342
Reactome: R-HSA-1250347
Reactome: R-HSA-1251985
Reactome: R-HSA-1257604
Reactome: R-HSA-177929
Reactome: R-HSA-179812
Reactome: R-HSA-180292
Reactome: R-HSA-180336
Reactome: R-HSA-182971
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642
Reactome: R-HSA-212718
Reactome: R-HSA-2179392
Reactome: R-HSA-2219530
Reactome: R-HSA-5336415
Reactome: R-HSA-5638303
Reactome: R-HSA-5673001
Reactome: R-HSA-6785631
Reactome: R-HSA-6811558
Reactome: R-HSA-8847993
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8857538
Reactome: R-HSA-8863795
Reactome: R-HSA-9009391
Reactome: R-HSA-9634638
Reactome: R-HSA-9664565
Reactome: R-HSA-9665686
Cellular Localisation Heparin-Binding Egf-Like Growth Factor: Secreted
Extracellular Space
Mature Hb-Egf Is Released Into The Extracellular Space And Probably Binds To A Receptor
Proheparin-Binding Egf-Like Growth Factor: Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth Factor antibody
Anti-Hb-Egf antibody
Anti-Hbegf antibody
Anti-Diphtheria Toxin Receptor antibody
Anti-Dt-R antibody
Anti-HBEGF antibody
Anti-DTR antibody
Anti-DTS antibody
Anti-HEGFL antibody

Information sourced from Uniprot.org

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