Anti-HCAR2 antibody (100-200) [S4625RM] (STJ11104625)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Hydroxycarboxylic Acid Receptor 2 (100-200) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4625RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HCAR2 |
| Gene ID | 338442 |
| Uniprot ID | HCAR2_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | CRLMLFMLAMNRQGSIIFLT VVAVDRYFRVVHPHHALNKI SNRTAAIISCLLWGITIGLT VHLLKKKMPIQNGGANLCSS FSICHTFQWHEAMFLLEFFL P |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GPR109A/HM74A/HCAR2 (NP_808219.1). |
Additional Info
| Tissue Specificity | Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils. |
| Function | Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. |
| Protein Name | Hydroxycarboxylic Acid Receptor 2G-Protein Coupled Receptor 109aG-Protein Coupled Receptor Hm74aNiacin Receptor 1Nicotinic Acid Receptor |
| Database Links | Reactome: R-HSA-3296197Reactome: R-HSA-373076Reactome: R-HSA-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Hydroxycarboxylic Acid Receptor 2 antibodyAnti-G-Protein Coupled Receptor 109a antibodyAnti-G-Protein Coupled Receptor Hm74a antibodyAnti-Niacin Receptor 1 antibodyAnti-Nicotinic Acid Receptor antibodyAnti-HCAR2 antibodyAnti-GPR109A antibodyAnti-HCA2 antibodyAnti-HM74A antibodyAnti-NIACR1 antibody |
Information sourced from Uniprot.org