• Western blot analysis of extracts of various cell lines, using HCRTR2 antibody (STJ23927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HCRTR2 antibody (380-444) (STJ23927)

SKU:
STJ23927

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCRTR2 (380-444) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HCRTR2
Gene ID: 3062
Uniprot ID: OX2R_HUMAN
Immunogen Region: 380-444
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-444 of human HCRTR2 (NP_001517.2).
Immunogen Sequence: SCCCLGVHHRQEDRLTRGRT STESRKSLTTQISNFDNISK LSEQVVLTSISTLPAANGAG PLQNW
Function Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.
Protein Name Orexin Receptor Type 2
Ox-2-R
Ox2-R
Ox2r
Hypocretin Receptor Type 2
Database Links Reactome: R-HSA-389397
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Orexin Receptor Type 2 antibody
Anti-Ox-2-R antibody
Anti-Ox2-R antibody
Anti-Ox2r antibody
Anti-Hypocretin Receptor Type 2 antibody
Anti-HCRTR2 antibody

Information sourced from Uniprot.org

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