Programmed Cell Death | Research Area | St John's Laboratory

Western blot detection of Hsp90 in Hela, 3T3, C6, K562 and A549 cell lysates using Hsp90 mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.

Anti-HSP90AA1 antibody [4B6-G2-H6] (STJ99220)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot detection of Hsp90 alpha in R Brain, Jurkat, CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Hsp90 alpha mouse mAb (1:1000 diluted). Predicted band size:90KDa. Observed band size:90KDa.

Anti-HSP90AA1 antibody [6H7-A3-2G6] (STJ99288)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Western blot analysis of lysates from Recombinant Human IL-1 alpha Protein (STJP000874) using IL-1 alpha Rabbit mAb (STJ11107358) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 10ng, 5ng Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-IL-1 alpha antibody (113-271 aa) [SRM5445] (STJ11107358)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Anti-IL-1 beta antibody [SM0462] (STJA0010462)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

£170.50 - £233.50

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Anti-IL-1 Beta antibody {ABflo® 488} (STJ11108624)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£0.00

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Anti-IL-1 Beta antibody {ABflo® 594} (STJ11108625)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£0.00

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Anti-IL1 Beta antibody [SRM13313] (STJ11108043)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human

£0.00

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Anti-IL1 Beta antibody [SRM14700] (STJ11109430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£0.00

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Anti-IL1 Beta antibody [SRM14856] (STJ11109586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Anti-IL18 antibody (1-193 aa) [SRM5148] (STJ11106987)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Anti-IL18 antibody (150-200) (STJ506215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-IL18 antibody (150-200) {Biotin} (STJ506216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-IL18 antibody (150-200) {FITC} (STJ506217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g IL18 antibody (STJ24170). Western blot was performed from the immunoprecipitate using IL18 antibody (STJ24170) at a dilution of 1:1000.

Anti-IL18 antibody (37-193) (STJ24170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-IL18 antibody (37-193) (STJ506218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Western blot analysis of various lysates, using IL18 antibody (STJ11104610) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL18 antibody (37-193) [S4610RM] (STJ11104610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Anti-IL18 antibody (37-193) {Biotin} (STJ506219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-IL18 antibody (37-193) {FITC} (STJ506220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IL18 antibody (STJ192030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-IL18 antibody [PT0148R] (STJA0039712)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£0.00

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Anti-IL18 antibody [SRM13359] (STJ11108089)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-IL18 antibody [SRM14586] (STJ11109316)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-IL18/IL1F4 antibody (Tyr37-Asp193) [1A151] (STJA0021976)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£229.50 - £328.50

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Anti-IL18/IL1F4 antibody [SAA0388] (STJA0027567)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-IL18/IL1F4 antibody [SAA0388] {APC} (STJA0029561)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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Anti-IL18/IL1F4 antibody [SAA0388] {FITC} (STJA0028595)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-IL18/IL1F4 antibody [SAA0388] {PerCP} (STJA0030527)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£265.50 - £396.50

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Anti-IL18/IL1F4 antibody [SAA0388] {PE} (STJA0031493)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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Western blot analysis of extracts of various cell lines, using (STJ24173) at 1:1000 dilution. Raw264. 7 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IL1A antibody (112-271) (STJ24173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of IL-1 alpha in paraffin-embedded human lung cancer using IL-1 alpha Rabbit polyclonal antibody (STJ113557) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-IL1A antibody (113-267) (STJ113557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-IL1A antibody (8-57 aa) (STJ93686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using IL-1 Alpha monoclonal antibody against truncated IL-1 Alpha recombinant protein.

Anti-IL1A antibody [1F3B3/3A6F11] (STJ98170)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-IL1A/IL1F1 antibody [SAA0366] (STJA0027545)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-IL1A/IL1F1 antibody [SAA0366] {APC} (STJA0028946)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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Anti-IL1A/IL1F1 antibody [SAA0366] {FITC} (STJA0027980)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-IL1A/IL1F1 antibody [SAA0366] {PerCP} (STJA0029912)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£265.50 - £396.50

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Anti-IL1A/IL1F1 antibody [SAA0366] {PE} (STJA0030878)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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Anti-IL1A/IL1F1 antibody [SAA0367] (STJA0027546)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-IL1A/IL1F1 antibody [SAA0367] {APC} (STJA0028947)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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Anti-IL1A/IL1F1 antibody [SAA0367] {FITC} (STJA0027981)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-IL1A/IL1F1 antibody [SAA0367] {PerCP} (STJA0029913)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£265.50 - £396.50

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Anti-IL1A/IL1F1 antibody [SAA0367] {PE} (STJA0030879)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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Western blot analysis of various lysates using IL1 Beta Rabbit pAb (STJ24174) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-IL1B antibody (1-269) (STJ24174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-IL-1β (PTR2541) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Untreated THP-1 Lane 2: THP-1 treated with LPS (100ng/ml) for 3hPredicted band size: 31, 18kDaObserved band size: 31, 18kDa

Anti-IL1B antibody (150-250) [PTR2541] (STJA0006256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, IL-1 Beta Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL1B antibody (181-230 aa) (STJ96566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from THP-1 cells, using IL1 Beta Rabbit polyclonal antibody (STJ118720) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-IL1B antibody (70-269) (STJ118720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-IL1B/IL1F2 antibody [SAA0368] (STJA0027547)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £341.50

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Anti-IL1B/IL1F2 antibody [SAA0368] {APC} (STJA0028948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £423.50

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