• Western blot analysis of various lysates, using Desmoplakin rabbit polyclonal antibody (STJ115263) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates, using Desmoplakin rabbit polyclonal antibody (STJ115263) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using Desmoplakin rabbit polyclonal antibody (STJ115263) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-DSP antibody (2609-2830) (STJ115263)

SKU:
STJ115263

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Desmoplakin (2609-2830) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DSP
Gene ID: 1832
Uniprot ID: DESP_HUMAN
Immunogen Region: 2609-2830
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2609-2830 of human Desmoplakin (NP_004406.2).
Immunogen Sequence: FSDTLEESSPIAAIFDTENL EKISITEGIERGIVDSITGQ RLLEAQACTGGIIHPTTGQK LSLQDAVSQGVIDQDMATRL KPAQKAFIGFEGVKGKKKMS AAEAVKEKWLPYEAGQRFLE FQYLTGGLVDPEVHGRISTE EAIRKGFIDGRAAQRLQDTS SYAKILTCPKTKLKISYKDA INRSMVEDITGLRLLEAASV SSKGLPSPYNMSSAPGSRSG SR
Tissue Specificity Expressed in oral mucosa (at protein level). Expressed in arrector pili muscle (at protein level). Isoform DPI: Apparently an obligate constituent of all desmosomes. Isoform DPII: Resides predominantly in tissues and cells of stratified origin.
Function Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance.
Protein Name Desmoplakin
Dp
250/210 Kda Paraneoplastic Pemphigus Antigen
Database Links Reactome: R-HSA-351906
Reactome: R-HSA-6798695
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Reactome: R-HSA-9696264
Reactome: R-HSA-9696273
Cellular Localisation Cell Junction
Desmosome
Cell Membrane
Localizes At The Intercalated Disk In Cardiomyocytes
Alternative Antibody Names Anti-Desmoplakin antibody
Anti-Dp antibody
Anti-250/210 Kda Paraneoplastic Pemphigus Antigen antibody
Anti-DSP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance