• Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Desmoplakin antibody (STJ29950) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Desmoplakin antibody (STJ29950) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human tonsil using Desmoplakin antibody (STJ29950) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Desmoplakin antibody (STJ29950) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DSP antibody (1-20) (STJ29950)

SKU:
STJ29950

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Desmoplakin (1-20) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DSP
Gene ID: 1832
Uniprot ID: DESP_HUMAN
Immunogen Region: 1-20
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-20 of human Desmoplakin (NP_004406.2).
Immunogen Sequence: MSCNGGSHPRINTLGRMIRA
Tissue Specificity Expressed in oral mucosa (at protein level). Expressed in arrector pili muscle (at protein level). Isoform DPI: Apparently an obligate constituent of all desmosomes. Isoform DPII: Resides predominantly in tissues and cells of stratified origin.
Function Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance.
Protein Name Desmoplakin
Dp
250/210 Kda Paraneoplastic Pemphigus Antigen
Database Links Reactome: R-HSA-351906
Reactome: R-HSA-6798695
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Reactome: R-HSA-9696264
Reactome: R-HSA-9696273
Cellular Localisation Cell Junction
Desmosome
Cell Membrane
Localizes At The Intercalated Disk In Cardiomyocytes
Alternative Antibody Names Anti-Desmoplakin antibody
Anti-Dp antibody
Anti-250/210 Kda Paraneoplastic Pemphigus Antigen antibody
Anti-DSP antibody

Information sourced from Uniprot.org

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