• Western blot analysis of extracts of various cell lines, using DSG1 antibody (STJ111854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of rat skin using DSG1 antibody (STJ111854) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat skin using DSG1 antibody (STJ111854) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DSG1 antibody (STJ111854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DSG1 antibody (800-1049) (STJ111854)

SKU:
STJ111854

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DSG1 (800-1049) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DSG1
Gene ID: 1828
Uniprot ID: DSG1_HUMAN
Immunogen Region: 800-1049
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 800-1049 of human DSG1 (NP_001933.2).
Immunogen Sequence: SPHFGTTTVISESTYPSGPG VLHPKPILDPLGYGNVTVTE SYTTSDTLKPSVHVHDNRPA SNVVVTERVVGPISGADLHG MLEMPDLRDGSNVIVTERVI APSSSLPTSLTIHHPRESSN VVVTERVIQPTSGMIGSLSM HPELANAHNVIVTERVVSGA GVTGISGTTGISGGIGSSGL VGTSMGAGSGALSGAGISGG GIGLSSLGGTASIGHMRSSS DHHFNQTIGSASPSTARSR
Tissue Specificity Epidermis, tongue, tonsil and esophagus.
Function Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Protein Name Desmoglein-1
Cadherin Family Member 4
Desmosomal Glycoprotein 1
Dg1
Dgi
Pemphigus Foliaceus Antigen
Database Links Reactome: R-HSA-351906
Reactome: R-HSA-6798695
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Desmosome
Cytoplasm
Nucleus
Alternative Antibody Names Anti-Desmoglein-1 antibody
Anti-Cadherin Family Member 4 antibody
Anti-Desmosomal Glycoprotein 1 antibody
Anti-Dg1 antibody
Anti-Dgi antibody
Anti-Pemphigus Foliaceus Antigen antibody
Anti-DSG1 antibody
Anti-CDHF4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance