Metabolism | Research Area | St John's Laboratory

Western blot analysis of S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody. The lane on the right is blocked with the S6 Ribosomal Protein (Phospho-Ser235+Ser236) peptide.

Anti-Phospho-RPS6-Ser235/S236 antibody (200-249 aa) (STJ90992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using S6 Ribosomal Protein (Phospho-Ser240) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6-Ser240 antibody (200-249 aa) (STJ91053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit mAb (STJ11107738) at 1:54000 dilution incubated overnight at 4C. HeLa, NIH/3T3 and PC-12 cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 30s.

Anti-Phospho-S6 Ribosomal Protein-S240/244 antibody [SRM] (STJ11107738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SAMHD1-Thr592 antibody (STJ196411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Syndecan4 (Phospho-Ser179) Antibody

Anti-Phospho-SDC4-Ser179 antibody (145-194 aa) (STJ91310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 3T3 cells, using Phospho-SP1-T739 Rabbit pAb (STJ22406). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SP1-T739 antibody (STJ22406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of HeLa cells, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SP1-Thr453 antibody (421-470 aa) (STJ90407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Sp1 (T739) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SP1-Thr739 antibody (706-755 aa) (STJ90408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-SPHK1-Ser-225 antibody (STJA0005597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£367.50

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Western Blot analysis of 1 MCF-7 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SPHK1-Ser225 antibody (STJA0009903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-SPHK2-Thr-578 antibody (STJA0005600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SPHK2 (Phospho-Thr614) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SPHK2-Thr614 antibody (580-629 aa) (STJ91268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using SREBP-1 (Phospho-Ser439) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SREBF1-Ser439 antibody (405-454 aa) (STJ90993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Western Blot analysis of HUVEC cell, HEK-293T cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SREBF2-Ser455 antibody (STJA0009941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-STK11-S334 Rabbit pAb (STJ29367) at 1:1000 dilution. Hela cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37 °C for 30 minutes after serum-starvation<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-STK11-S334 antibody (STJ29367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STK11-S428 Rabbit pAb (STJ11101142) at 1:1000 dilution or LKB1 antibody (STJ25732). NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-STK11-S428 antibody (STJ11101142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-STK11-S428 antibody (STJ29368). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-STK11-S428 antibody (STJ29368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-STK11-Ser334 antibody (300-349 aa) (STJ91208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, LKB1 (phospho Ser428) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-STK11-Ser428 antibody (384-433 aa) (STJ91060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Cow

£48.00 - £252.00

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Western blot analysis of lysates from A-431 cells, using Phospho-STK11-T189 Rabbit pAb (STJ25732). A-431 cells were treated by 10% FBS for after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit

Anti-Phospho-STK11-T189 antibody (STJ29369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of 3T3 cells using Phospho-LKB1 (T189) Polyclonal Antibody

Anti-Phospho-STK11-Thr189 antibody (155-204 aa) (STJ90488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-STX1A-Ser14 antibody (1-50 aa) (STJ90729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using MUNC-18a (Phospho-Ser313) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-STXBP1-Ser313 antibody (279-328 aa) (STJ90471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from PC-12 cells, using Phospho-Tyrosine Hydroxylase-S40 pAb (STJ11101143) at 1:1000 dilution or Tyrosine Hydroxylase antibody (STJ29777). PC-12 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-TH-S40 antibody (STJ11101143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-TH (S19) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TH-Ser19 antibody (10-59 aa) (STJ91023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-TH (S62) Polyclonal Antibody

Anti-Phospho-TH-Ser62 antibody (1-50 aa) (STJ90748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-TH (S71) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TH-Ser71 antibody (41-90 aa) (STJ90749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells treated with paclitaxel 1uM 24h, using TK (Phospho-Ser13) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TK1-Ser13 antibody (1-50 aa) (STJ90834)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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Anti-Phospho-TNFRSF21-Ser562 antibody (STJ196287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Tryptophan Hydroxylase (Phospho-Ser260) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TPH1-Ser260 antibody (231-280 aa) (STJ90908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-TPH1 (S58) Polyclonal Antibody

Anti-Phospho-TPH1-Ser58 antibody (26-75 aa) (STJ90907)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HepG2 cells using Phospho-TPH2 (S19) Polyclonal Antibody

Anti-Phospho-TPH2-Ser19 antibody (STJ90661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Tyrosine Hydroxylase-S19 antibody (STJ11107634)

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from 293 cells, using Phospho-Tyrosine Hydroxylase-S31 Rabbit pAb (STJ11107621). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Tyrosine Hydroxylase-S31 antibody (STJ11107621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HT29 cells treated with heat shock, using Vitamin D Receptor (Phospho-Ser208) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VDR-Ser208 antibody (181-230 aa) (STJ90730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of COLO cells using Phospho-VDR (S51) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-VDR-Ser51 antibody (16-65 aa) (STJ90600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-WASL-Ser-484/Ser-485 antibody (STJA0005647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-WASL-Tyr-256 antibody (STJA0005646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-PHYH antibody (1-338 aa) (STJ11106103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PHYH antibody (STJ28226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PHYH antibody (1-338) (STJ28226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-PHYH antibody (STJA0007318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 and HepG2 cells, using AGXT2L2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PHYKPL antibody (341-390 aa) (STJ91509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat spleen using PI3 Kinase p110 delta Rabbit monoclonal antibody (STJ11103107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PI3 Kinase p110 delta antibody (200-300) [S7MR] (STJ11103107)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using PI3 Kinase p85 alpha Mouse mAb (STJ11106530) at 1:1000 dilution. <br/> Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 30s.

Anti-PI3 Kinase p85 alpha antibody [SMM] (STJ11106530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PI3R6 antibody (590-640) (STJ506050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£427.50

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Anti-PI3R6 antibody (590-640) {Biotin} (STJ506051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-PI3R6 antibody (590-640) {FITC} (STJ506052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Western blot analysis of lysates from HeLa cells, using PI4K2A Rabbit polyclonal antibody (STJ118973) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PI4K2A antibody (131-479) (STJ118973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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