Anti-MED25 antibody (30-200) (STJ118590)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MED25 (30-200) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MED25 |
| Gene ID | 81857 |
| Uniprot ID | MED25_HUMAN |
| Immunogen Region | 30-200 |
| Immunogen Sequence | PYFEGLRKHYLLPAIEYFNG GPPAETDFGGDYGGTQYSLV VFNTVDCAPESYVQCHAPTS SAYEFVTWLDGIKFMGGGGE SCSLIAEGLSTALQLFDDFK KMREQIGQTHRVCLLICNSP PYLLPAVESTTYSGCTTENL VQQIGERGIHFSIVSPRKLP ALRLLFEKAAP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human MED25 (NP_112235.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen. |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription. |
| Protein Name | Mediator Of Rna Polymerase Ii Transcription Subunit 25Activator Interaction Domain-Containing Protein 1Activator-Recruited Cofactor 92 Kda ComponentArc92Mediator Complex Subunit 25P78 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-212436Reactome: R-HSA-381340Reactome: R-HSA-9833110 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 25 antibodyAnti-Activator Interaction Domain-Containing Protein 1 antibodyAnti-Activator-Recruited Cofactor 92 Kda Component antibodyAnti-Arc92 antibodyAnti-Mediator Complex Subunit 25 antibodyAnti-P78 antibodyAnti-MED25 antibodyAnti-ACID1 antibodyAnti-ARC92 antibodyAnti-PTOV2 antibodyAnti-TCBAP0758 antibody |
Information sourced from Uniprot.org