| Short Description : | Rabbit polyclonal MED20 (1-212) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MED20 |
| Gene ID: | 9477 |
| Uniprot ID: | MED20_HUMAN |
| Immunogen Region: | 1-212 |
| Immunogen Sequence: | MGVTCVSQMPVAEGKSVQQT VELLTRKLEMLGAEKQGTFC VDCETYHTAASTLGSQGQTG KLMYVMHNSEYPLSCFALFE NGPCLIADTNFDVLMVKLKG FFQSAKASKIETRGTRYQYC DFLVKVGTVTMGPSARGISV EVEYGPCVVASDCWSLLLEF LQSFLGSHTPGAPAVFGNRH DAVYGPADTMVQYMELFNKI RKQQQVPVAGIR |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_004266.2). |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Protein Name | Mediator Of Rna Polymerase Ii Transcription Subunit 20Mediator Complex Subunit 20Trf-Proximal Protein HomologHtrfp |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-212436Reactome: R-HSA-381340Reactome: R-HSA-9833110Reactome: R-HSA-9841922 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 20 antibodyAnti-Mediator Complex Subunit 20 antibodyAnti-Trf-Proximal Protein Homolog antibodyAnti-Htrfp antibodyAnti-MED20 antibodyAnti-TRFP antibody |
Information sourced from Uniprot.org
