• Immunohistochemistry analysis of MED4 in paraffin-embedded rat colon tissue using MED4 Rabbit monoclonal antibody (STJ11102700) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MED4 in paraffin-embedded human colon tissue using MED4 Rabbit monoclonal antibody (STJ11102700) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MED4 in paraffin-embedded human esophagus tissue using MED4 Rabbit monoclonal antibody (STJ11102700) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MED4 in paraffin-embedded mouse colon tissue using MED4 Rabbit monoclonal antibody (STJ11102700) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MED4 in paraffin-embedded human breast tissue using MED4 Rabbit monoclonal antibody (STJ11102700) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MED4 in paraffin-embedded human breast cancer tissue using MED4 Rabbit monoclonal antibody (STJ11102700) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MED4 Rabbit monoclonal antibody (STJ11102700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MED4 antibody (150-250) [S0MR] (STJ11102700)

SKU:
STJ11102700

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MED4 (150-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MED4
Gene ID: 29079
Uniprot ID: MED4_HUMAN
Immunogen Region: 150-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human MED4 (Q9NPJ6).
Immunogen Sequence: KYAHRISASNAVCAPLTWVP GDPRRPYPTDLEMRSGLLGQ MNNPSTNGVNGHLPGDALAA GRLPDVLAPQYPWQSNDMSM NMLPPNHSSDFLLEPPGHNK E
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Protein Name Mediator Of Rna Polymerase Ii Transcription Subunit 4
Activator-Recruited Cofactor 36 Kda Component
Arc36
Mediator Complex Subunit 4
Trap/Smcc/Pc2 Subunit P36 Subunit
Vitamin D3 Receptor-Interacting Protein Complex 36 Kda Component
Drip36
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-212436
Reactome: R-HSA-381340
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 4 antibody
Anti-Activator-Recruited Cofactor 36 Kda Component antibody
Anti-Arc36 antibody
Anti-Mediator Complex Subunit 4 antibody
Anti-Trap/Smcc/Pc2 Subunit P36 Subunit antibody
Anti-Vitamin D3 Receptor-Interacting Protein Complex 36 Kda Component antibody
Anti-Drip36 antibody
Anti-MED4 antibody
Anti-ARC36 antibody
Anti-DRIP36 antibody
Anti-VDRIP antibody
Anti-HSPC126 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance