Metabolism | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using HSD17B13 antibody (STJ28012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HSD17B13 antibody (30-211) (STJ28012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of extracts of various cell lines, using HSD17B13 antibody (STJ11104159) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HSD17B13 antibody (30-211) [S4159RM] (STJ11104159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Immunofluorescence analysis of paraffin-embedded Human liver using HSD17B13 Rabbit polyclonal antibody (STJ11105579) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSD17B13 antibody (34-300) (STJ11105579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

€138.13 - €631.88

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HSD17B14 antibody (STJ190741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of various lysates, using HSD17B2 antibody (STJ24083) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 1s.

Anti-HSD17B2 antibody (100-387) (STJ24083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HSD17B2 antibody (STJ190811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Anti-HSD17B2 antibody [SRM14974] (STJ11109704)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with HSD17B3 using HSD17B3 Rabbit polyclonal antibody (STJ110000) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSD17B3 antibody (1-310) (STJ110000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-HSD17B3 antibody (STJA0007544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of HuvEc, K562 cells using 17 Beta-HSD4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HSD17B4 antibody (41-90 aa) (STJ96751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of HSD17B7 in paraffin-embedded Human breast cancer using HSD17B7 Rabbit polyclonal antibody (STJ11102864) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-HSD17B7 antibody (110-200) (STJ11102864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Anti-HSD17B7 antibody (STJA0007807)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of U-2 OS cells using HSD17B8 Rabbit polyclonal antibody (STJ114813) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSD17B8 antibody (1-261) (STJ114813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HSD17B8 antibody (STJ190812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of various lysates using HSD3B1 Rabbit pAb (STJ110340) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HSD3B1 antibody (1-290) (STJ110340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunohistochemistry analysis of HSD3B1 in paraffin-embedded human placenta using HSD3B1 Rabbit monoclonal antibody (STJ11102997) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-HSD3B1 antibody (250-350) [S7MR] (STJ11102997)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HSD3B1 antibody (30-110 aa) (STJ190453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of A-549 cells using HSD3B2 Rabbit polyclonal antibody (STJ26181). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-HSD3B2 antibody (1-287) (STJ26181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Anti-HSD3B2 antibody (180-260 aa) (STJ190454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using 3 Beta-HSD7 Polyclonal Antibody diluted at 1：1000

Anti-HSD3B7 antibody (121-170 aa) (STJ91380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-HSL/LIPE antibody [SRM14764] (STJ11109494)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€0.00

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Anti-Hsp90 Alpha antibody [SRM14914] (STJ11109644)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-Hsp90 Beta antibody [SRM14646] (STJ11109376)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Rice

€0.00

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Hsp90 Alpha antibody (STJ11102219). Western blot was performed from the immunoprecipitate using Hsp90 Alpha antibody (STJ11102219) at a dilution of 1:1000.

Anti-HSP90AA1 antibody (1-100) [S9MR] (STJ11102219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€179.38 - €773.13

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Western blot analysis of various lysates, using HSP90AA1 Rabbit polyclonal antibody (STJ11105239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AA1 antibody (433-732 aa) (STJ11105239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Immunofluorescence analysis of U2OS cells using Hsp90 Alpha Rabbit polyclonal antibody (STJ24087). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-HSP90AA1 antibody (433-732) (STJ24087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of HSP90 in K562, C6, Hela lysates using Hsp90 alpha antibody.

Anti-HSP90AA1 antibody (62-75 aa) [R06-9C1] (STJA0010746)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

€330.63

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Western blot analysis of various lysates using Hsp90 Alpha Rabbit pAb (STJ114322) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HSP90AA1 antibody (629-732) (STJ114322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSP90 a Mouse mAb diluted at 1:2, 000.

Anti-HSP90AA1 antibody (660-740 aa) [1F6] (STJ97496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of Hela cell. 1, HSP90A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP90AA1 antibody (683-732 aa) (STJ93622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot detection of Hsp90 in Hela, 3T3, C6, K562 and A549 cell lysates using Hsp90 mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.

Anti-HSP90AA1 antibody [4B6-G2-H6] (STJ99220)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€186.25 - €450.00

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Western blot detection of Hsp90 alpha in R Brain, Jurkat, CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Hsp90 alpha mouse mAb (1:1000 diluted). Predicted band size:90KDa. Observed band size:90KDa.

Anti-HSP90AA1 antibody [6H7-A3-2G6] (STJ99288)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

€186.25 - €450.00

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Immunofluorescence analysis of U2OS cells using Hsp90 Beta Rabbit polyclonal antibody (STJ11104548). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSP90AB1 antibody (1-300) (STJ11104548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from wild type (WT) and HSP90AB1 knockout (KO) HeLa cells, using [KO Validated] Hsp90 Beta Rabbit polyclonal antibody (STJ119988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AB1 antibody (1-300) (STJ119988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€158.13 - €773.13

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Immunohistochemistry analysis of Hsp90 Beta in paraffin-embedded Human small intestine tissue using [KO Validated] Hsp90 Beta Rabbit polyclonal antibody (STJ24088) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-HSP90AB1 antibody (1-300) (STJ24088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of various cells using HSP90 Beta Polyclonal Antibody diluted at 1：2000

Anti-HSP90AB1 antibody (226-275 aa) (STJ93609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Western blot detection of Hsp90 beta in Hela, 3T3, C6, CHO-K1 and COS7 cell lysates using Hsp90 beta mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.

Anti-HSP90AB1 antibody [3B9-D4-G4] (STJ99221)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat/Monkey/Hamster

€186.25 - €450.00

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Western blot analysis using HSP90 Beta monoclonal antibody against Jurkat (1) , A431 (2) , HeLa (3) , A549 (4) , HEK293 (5) , K562 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-HSP90AB1 antibody [5G4] (STJ98144)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€186.25 - €450.00

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Western blot analysis of extracts of various cell lines, using Grp75/MOT/HSPA9 Rabbit monoclonal antibody (STJ11101306) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HSPA9 antibody (580-679) [S6MR] (STJ11101306)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Anti-HSPG2 antibody (STJA0004402)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

€175.63 - €519.38

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Anti-HSPG2 antibody (STJA0007971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Anti-HSPG2 antibody [SAA1656] (STJA0020084)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€426.88 - €1,809.38

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Immunofluorescence analysis of PC-12 cells using HYAL1 Rabbit polyclonal antibody (STJ11105720) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HYAL1 antibody (136-435) (STJ11105720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysates, using HYAL1 Rabbit monoclonal antibody (STJ11105225) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : MOLT-4Exposure time: 60s.

Anti-HYAL1 antibody (22-435) [S5225RM] (STJ11105225)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Anti-HYAL1 antibody (STJA0009333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-HYAL2 antibody (STJA0007479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-HYAL3 antibody (STJA0008263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-HYAL4 antibody (STJA0008905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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